The cusk eel Genypterus chilensis (Guichenot, 1848) is a fish demanded by the Chilean market. Their catches have remained under 1,000 ton per year in the decade 2000-2010, at a price of US$7 kg(-1). The aim of this study was to evaluate the growth of first generation juveniles produced by wild pa...
Vega Aguayo, Rolando
,
Manuel Estrada, Juan
,
Ramirez, Diego
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Flores, Camila
,
Zamorano, Jose
,
Encina Montoya, Francisco
,
Mardones Lazcano, Alfonso
,
Valdebenito Isler, Iván
,
Dantagnan Dantagnan, Patricio
,
[Growth of cusk eel Genypterus chilensis juveniles in culture conditions]
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Growth of cusk eel Genypterus chilensis juveniles in culture conditions
The nuclear DNA content (2C value) was determined in the commercial fish Galaxias maculatus (Galaxiidae) was determined by microdensitometry of erythrocyte nuclei after Feulgen staining; rainbow trout erythrocytes with a known 2C value were used as a standard. The 2C value of G. maculatus was 2.2...
This study was designed to test a vitrification method on spermatozoa of sex-reversed rainbow trout and determine the ability of sucrose, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA) and seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium comprised a st...
Figueroa Villalobos, Elías
,
Risopatron, J.
,
Sanchez, R.
,
Isachenko, E.
,
Merino, O.
,
Isachenko, V.
,
Valdebenito Isler, Iván
,
[Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters]
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Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters
Storage of refrigerated semen is a simple and inexpensive procedure that can facilitate the management and reproduction programmes in aquaculture and allows to store semen for in vitro reproduction, quality analyses, hatchery production support, selective in vitro breeding, disease diagnosis, tra...
Contreras, Pablo
,
Dumorne, Kelly
,
Ulloa-Rodriguez, Patricio
,
Merino, Osvaldo
,
Figueroa Villalobos, Elías
,
Farias, Jorge G.
,
Valdebenito Isler, Iván
,
Risopatron, Jennie
,
[Effects of short-term storage on sperm function in fish semen: a review]
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Effects of short-term storage on sperm function in fish semen: a review
Patagonian blenny (E. maclovinus) is a marine species recently placed in captivity and which are potentially farmable. Understanding and improving its sperm capacity to withstand short-term storage conditions is a key element of initiating an artificial propagation program for this species. The a...
Ulloa Rodriguez, Patricio
,
Contreras, Pablo
,
Dumorne, Kelly
,
Lee Estevez, Manuel
,
Diaz, Rommy
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Risopatron, Jennie
,
Farias, Jorge G.
,
[Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium]
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Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium
Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...
The aims of this investigation were to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified u...
Merino, O.
,
Sanchez, R.
,
Risopatron, J.
,
Isachenko, E.
,
Katkov, I. I.
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Mallmann, P.
,
Isachenko, V.
,
[Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report]
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Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report
The development of semen cryopreservation strategies is necessary to improve the semen storage technologies of species of great commercial interest for aquaculture. Recent studies demonstrate that lipids play an important role in the fertility and cryotolerance of fish gametes. This study investi...
Spermatological research of the Patagonian blennie was carried, specifically biometric parameters, sperm density, sperm count and motility in different activation mediums (815, 716, 590 and 0mOSmkg(-1)), at different temperatures (5, 10 and 15 degrees C) and pH levels (5, 7 and 9). The results in...
Valdebenito Isler, Iván
,
Cosson, J.
,
Contreras, P.
,
Sanchez, J. C.
,
Oliveira, R. P. S.
,
Risopatron, J.
,
Farias, J. G.
,
Figueroa Villalobos, Elías
,
[Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile]
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Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile
In the following investigation the morphometric characteristics of the first two blastomeres of rainbow trout (Oncorhynchus mykiss) were determined. Embryos were incubated at 9 degrees C and then fixed in a Stockard solution every 30 min starting from 8.5 to 12.5 h of incubation post fertilizatio...
Valdebenito Isler, Iván
,
Sanchez, Ruben R.
,
Effer Roldan, Brian
,
Ubilla Madrid, Andrea
,
[Morphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss)]
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Morphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss)