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In this study, the morphology and ultrastructure of Genypterus blacodes spermatozoa were characterized through scanning and transmission electron microscopy. Findings revealed that the G. blacodes spermatozoa can be differentiated into three major parts: a spherical head without an acrosome (typi...


Dumorne, KellyValdebenito Isler, IvánRisopatron, JennieFigueroa Villalobos, ElíasDiaz, RommyFarias, Jorge[Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy]Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy

The Zebrafish (Danio Rerio) is a Teleost fish, which offers several practical advantages concerning manipulation, maintenance and versatility as well as short life cycle, low cost and physiological similarity with most farmed species. Zebrafish have been used as model for several aquaculture-rele...


Lee Estevez, ManuelFigueroa Villalobos, ElíasCosson, JackyShort, Stefania E.Valdebenito Isler, IvánUlloa Rodriguez, PatricioFarias, Jorge G.[Zebrafish as a useful model for immunological research with potential applications in aquaculture]Zebrafish as a useful model for immunological research with potential applications in aquaculture
Dumorne, KellyValdebenito Isler, IvánContreras, PabloUlloa Rodriguez, PatricioRisopatron, JennieFigueroa Villalobos, ElíasEstevez, Manuel LeeDiaz, RommyFarias, Jorge[Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))]Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))

This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....


Figueroa Villalobos, ElíasMerino, O.Risopatron, J.Isachenko, V.Sanchez, R.Effer Roldan, BrianIsachenko, E.Farias, J. G.Valdebenito Isler, Iván[Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification]Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification

The use of synthetic hormones to regulate sexual maturity in captive fish is a common practice. With aquaculture practices, fish production is desired throughout the year, necessitating the maintenance of quality standards, mainly regarding the characteristics of the fish produced. Embryonic deve...


Zepeda, Andrea B.Miranda, Ignacia B.Valdebenito Isler, IvánMoreno, Ricardo D.Farias, Jorge G.[GnRHa treatments of Atlantic Salmon broodstock suppresses effects of endocrine disruptors, benefitting offspring quality]GnRHa treatments of Atlantic Salmon broodstock suppresses effects of endocrine disruptors, benefitting offspring quality

The development of cryopreservation techniques has led to important changes in animal reproductive biotechnology. However, these techniques are associated with cellular and molecular damage, affecting the mitochondrial function and quality of spermatozoa; moreover studies in fish are limited. In ...


Figueroa Villalobos, ElíasLee Estevez, M.Valdebenito Isler, IvánWatanabe, IOliveira, R. P. S.Romero, J.Castillo, R. L.Farias, J. G.[Effects of cryopreservation on mitochondrial function and sperm quality in fish]Effects of cryopreservation on mitochondrial function and sperm quality in fish

Calcium is a prerequisite factor for triggering the sperm activation in salmonids. The aim of this study was to test different concentrations of Ca(2+)on sperm motility activation and fertility of rainbow trout (Oncorhynchus mykiss). Semen samples activated with calcium free activator (0.0 mM) an...


Sandoval Vargas, LeydyFigueroa Villalobos, EliasContreras, PabloEstay, FranciscoValdebenito Isler, Iván[Effect of different calcium concentration on sperm motility and fertilisation capacity of rainbow trout (Oncorhynchus mykiss)]Effect of different calcium concentration on sperm motility and fertilisation capacity of rainbow trout (Oncorhynchus mykiss)

Patagonian blenny (Eleginops maclovinus) is the only member of Eleginopidae family. It has been an important resource for Chilean artisanal fishermen communities, however, capture volumes have progressively decreased over last decades likely because overfishing, and concerns for conservation of t...


Lee-Estevez, ManuelLarama, GiovanniFigueroa Villalobos, ElíasUlloa-Rodriguez, PatricioDiaz, RommyValdebenito Isler, IvánFarias, Jorge G.[Complete mitochondrial genome sequence of Patagonian blenny, Eleginops maclovinus (Perciformes: Eleginopidae) with phylogenetic consideration]Complete mitochondrial genome sequence of Patagonian blenny, Eleginops maclovinus (Perciformes: Eleginopidae) with phylogenetic consideration

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