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In teleostei, as opposed to what happens in mammals, most of the studies that evaluate the quality storages of semen are oriented toward the exposure of spermatozoa to some reactive oxygen species (ROS), the utilization of antioxidants in the diet, or the incorporation of these in seminal plasma....


Berrios, O.Valdebenito Isler, IvánTreulen, F.Ubilla Madrid, Andrea[Cold storage of sperm of rainbow trout (Oncorhynchus mykiss): Effect on motility, intracellular superoxide, plasma membrane integrity and mitochondrial membrane potential]Cold storage of sperm of rainbow trout (Oncorhynchus mykiss): Effect on motility, intracellular superoxide, plasma membrane integrity and mitochondrial membrane potential

In fish feeding studies, stomach related parameters are mainly measured in order to have biometric relationships. To determine those parameters a direct and straightforward method for measuring real maximum stomach volume in fish based on Archimedes' principle was tested and compared against a tr...


Salgado Ismodes, AndresSalgado Leu, ItaloValdebenito Isler, Iván[A direct and straightforward method for measurement real maximum fish stomach volume to improve aquaculture feeding research]A direct and straightforward method for measurement real maximum fish stomach volume to improve aquaculture feeding research

The present investigation determined how different antioxidants incorporated into the sperm diluent for cold storage of semen affected sperm motility and spermatozoan fertility capabilities of the rainbow trout. For the evaluations, fresh semen (C) and semen that had been stored without diluents ...


Ubilla Madrid, AndreaValdebenito Isler, Iván[Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability]Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability

The quality of fish gametes, both male and female, are determined by several factors (age, management, feeding, chemical and physical factors, water quality, etc.)that have an impact on the survivability of embryos, larvae and/or fry in the short or long term. One of the most important factors is...


Valdebenito Isler, IvánGallegos, Patricia C.Effer Roldan, Brian[Gamete quality in fish: evaluation parameters and determining factors]Gamete quality in fish: evaluation parameters and determining factors

In this article we describe basic aspects of the sperm biology of Merluccius australis, in particular assessing the effects of pH (6, 8 and 10), temperature (4, 10 and 15 degrees C) and osmolality (922 mOsm/kg, 822 mOsm/kg, 681 mOsm/kg and 3 mOsm/kg) on sperm motility. Our results indicate the pr...


Effer Roldan, BrianFigueroa Villalobos, ElíasAugsburger, A.Valdebenito Isler, Iván[Sperm biology of Merluccius australis: Sperm structure, semen characteristics and effects of pH, temperature and osmolality on sperm motility]Sperm biology of Merluccius australis: Sperm structure, semen characteristics and effects of pH, temperature and osmolality on sperm motility

Sperm cryopreservation is an important tool for reproductive management; however, it has not been possible to perform this procedure for coho salmon. This study was designed to assess the effect of cryopreservation on sperm functional parameters, oxidative stress markers and fertilizing capacity ...


Sandoval Vargas, LeydyDumorne, KellyContreras, PabloFarias, Jorge G.Figueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, Iván[Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity]Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...


Figueroa Villalobos, ElíasValdebenito Isler, IvánMerino, O.Ubilla Madrid, AndreaRisopatron, J.Farias, J. G.[Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology]Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...


Risopatron, J.Merino, O.Cheuqueman, C.Figueroa Villalobos, ElíasSanchez, R.Farias, J. G.Valdebenito Isler, Iván[Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)]Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)

The current trend in world aquaculture is towards the diversification of cultures, mainly native species. The red cusk eel Genypterus chilensis is a native Chilean species of high gastronomic demand and seasonal exploitation that is projected as a candidate for the development of farming technolo...


Vega Aguayo, RolandoPradenas, MiguelManuel Estrada, JuanRamirez, DiegoValdebenito Isler, IvánMardones Lazcano, AlfonsoDantagnan Dantagnan, PatricioAlfaro, DenisEncina Montoya, FranciscoPichara Morales, Cristian[Evaluation and comparison of the efficiency of two incubation systems for Genypterus chilensis (Guichenot, 1848) eggs]Evaluation and comparison of the efficiency of two incubation systems for Genypterus chilensis (Guichenot, 1848) eggs

In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viabilit...


Ubilla Madrid, AndreaValdebenito Isler, IvánArias, M. E.Risopatron, J.[Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C]Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C

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