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The distribution and frequency of single nucleotide polymorphisms (SNPs) is an excellent tool for discerning evolutionary relatedness between cultivated and wild plant genomes. This type of information is scanty for the genus Manihot, and thus limiting systematic approaches in the genetic improve...


Beyene, D.Mukasa, S.B.Jansson, C.Villiers, Etienne P. deFerguson, M.Rabbi, Ismail Y.Kyamanywa, S.Sebuliba, J.Baguma, Yona K.[Patterns on nucleotide diversity in Meisa1 and G3PHD in wild and cultivated cassava]Patterns on nucleotide diversity in Meisa1 and G3PHD in wild and cultivated cassava

Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73%...


Bishop, Richard P.Shah, TushaarPelle, RogerHoyle, D.Pearson, T.Haines, L.Brass, A.Hulme, H.Graham, S.P.Taracha, E.L.N.Lu, C.Hass, B.Wortman, J.White, O.Gardner, M.J.Nene, VishvanathVilliers, Etienne P. de[Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage]Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

Heartwater, a tick-borne disease of domestic and wild ruminants, is caused by the intracellular rickettsia Ehrlichia ruminantium (previously known as Cowdria ruminantium). It is a major constraint to livestock production throughout subSaharan Africa, and it threatens to invade the Americas, yet t...


Collins, N.E.Liebenberg, J.Villiers, Etienne P. deBrayton, K.A.Louw, E.Pretorius, E.Faber, F.E.Heerden, H. vanJosemans, A.Kleef, M. vanSteyn, H.C.Strijp, M.F. vanZweygarth, E.Jongejan, F.Maillard, J.C.Berthier, D.Botha, M.Joubert, F.Corton, C.H.Thomson, N.R.Allsopp, M.T.Allsopp, B.A.[The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number]The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number

Transcriptome analysis can provide useful data for refining genome sequence annotation. Application of massively parallel signature sequencing (MPSS) revealed reproducible transcription, in multiple MPSS cycles, from 73% of computationally predicted genes in the Theileria parva schizont lifecycle...


Shah, TushaarVilliers, Etienne P. deNene, VishvanathHass, B.Taracha, E.A.Gardner, J.M.Sansom, C.Pelle, RogerBishop, Richard P.[Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)]Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)

Pain, A.Renauld, H.Berriman, M.Murphy, L.Yeats, C.A.Weir, W.Kerhornou, A.Aslett, M.Bishop, Richard P.Bouchier, C.Cochet, M.Coulson, R.M.R.Cronin, A.Villiers, Etienne P. deFraser, A.Fosker, N.Gardner, M.Goble, A.Griffiths-Jones, S.Harris, D.E.Katzer, F.Larke, N.Lord, A.Maser, P.McKellar, S.Mooney, P.Morton, F.Nene, VishvanathO'Neil, S.Price, C.Quail, M.A.Rabbinowitsch, E.Rawlings, N.D.Rutter, S.Saunders, D.Seeger, K.Shah, TushaarSquares, R.Squares, S.Tivey, A.Walker, A.R.Woodward, J.Dobbelaere, D.A.E.Langsley, G.Rajandream, M.A.McKeever, Declan J.Shiels, B.Tait, A.Barrell, B.Hall, N.[Genome of the host-cell transforming parasite Theileria annulata compared with T. parva]Genome of the host-cell transforming parasite Theileria annulata compared with T. parva

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from tic...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-i...


Graham, S.P.Pelle, RogerHonda, Y.Mwangi, D.M.Tonukari, N.J.Yamage, M.Glew, E.J.Villiers, Etienne P. de[Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes]Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes

Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...


Sunter, J.D.Patel, S.P.Skilton, Robert A.Knowles, Donald P.Scoles, Glen A.Nene, VishvanathVilliers, Etienne P. deBishop, Richard P.[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata

The published genomic sequences of the two major host-transforming Theileria species of cattle represent a rich resource of information that has allowed novel bioinformatic and experimental studies into these important apicomplexan parasites. Since their publication in 2005, the genomes of T. ann...


Weir, W.Sunter, J.Chaussepied, M.Skilton, Robert A.Tait, A.Villiers, Etienne P. deBishop, Richard P.Shiels, B.Langsley, G.[Highly syntenic and yet divergent: a tale of two Theilerias]Highly syntenic and yet divergent: a tale of two Theilerias

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