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Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73%...


Bishop, Richard P.Shah, TushaarPelle, RogerHoyle, D.Pearson, T.Haines, L.Brass, A.Hulme, H.Graham, S.P.Taracha, E.L.N.Lu, C.Hass, B.Wortman, J.White, O.Gardner, M.J.Nene, VishvanathVilliers, Etienne P. de[Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage]Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated through the destruction of parasite infected lymphocytes by MHC class I restricted CD8+ CTL. Six parasite proteins targeted by CTL from T. parva immune cattle have been identified and raised...


Graham, S.P.Pelle, RogerVilliers, Etienne P. deYamage, M.Mwangi, D.M.Honda, Y.Ellis, S.A.MacHugh, Niall D.Morrison, W. IvanTaracha, E.L.N.[Identification of bovine cytotoxic T lymphocyte epitopes on the intracellular parasite Theileria Parva]Identification of bovine cytotoxic T lymphocyte epitopes on the intracellular parasite Theileria Parva
Gardner, M.J.Bishop, Richard P.Shah, TushaarVilliers, Etienne P. deCarlton, J.M.Hall, N.Ren, Q.Paulsen, I.T.Pain, A.Berriman, M.Wilson, R.J.M.Sato, S.Ralph, S.A.Mann, D.J.Xiong, Z.Shallom, S.J.Weidman, J.Jiang, L.Lynn,, J.Weaver, B.Shoaibi, A.Domingo, A.R.Wasawo, D.Crabtree, J.Wortman, J.R.Haas, B.Angiuoli, S.V.Creasy, T.H.Lu, C.Suh, B.Silva, J.C.Utterback, T.R.Feldblyum, T.V.Pertea, M.Allen, J.Nierman, W.C.Taracha, E.L.N.Salzberg, S.L.White, O.R.Fitzhugh, H.A.Morzaria, S.P.Venter, J. CraigFraser, C.M.Nene, Vishvanath[Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes]Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes
Pain, A.Renauld, H.Berriman, M.Murphy, L.Yeats, C.A.Weir, W.Kerhornou, A.Aslett, M.Bishop, Richard P.Bouchier, C.Bouchier, C.Cochet, M.Coulson, R.M.R.Cronin, A.Villiers, Etienne P. deFraser, A.Fosker, N.Gardner, M.Goble, A.Griffiths-Jones, S.Harris, D.E.Katzer, F.Larke, N.Lord, A.Maser, P.McKellar, S.Mooney, P.Morton, F.Nene, VishvanathO'Neil, S.Price, C.Quail, M.A.Rabbinowitsch, E.Rawlings, N.D.Rutter, S.Saunders, D.Seeger, K.Shah, TushaarSquares, R.Squares, S.Tivey, A.Walker, A.R.Woodward, J.Dobbelaere, D.A.E.Langsley, G.Rajandream, M.A.McKeever, Declan J.Shiels, B.Tait, A.Barrell, B.Hall, N.[Genome of the host-cell transforming parasite Theileria annulata compared with T. parva]Genome of the host-cell transforming parasite Theileria annulata compared with T. parva

Tick salivary gland proteins are responsible for suppression/modulation of host immune responses and subsets of such genes whose expression is modified by Theileria parva infection would be candidates for inclusion in anti-tick/anti-pathogen combination vaccines. We used subtractive hybridisation...


Obura, M.Skilton, Robert A.Villiers, Etienne P. deKutima, H.Bishop, Richard P.[Application of suppressive subtractive hybridisation technique to isolate differentially expressed genes in Rhipicephalus appendiculatus ticks following infection by Theileria parva]Application of suppressive subtractive hybridisation technique to isolate differentially expressed genes in Rhipicephalus appendiculatus ticks following infection by Theileria parva
Mwangi, D.M.Graham, S.P.Pelle, RogerHonda, Y.Tonukari, N.J.Yamage, I.M.Glew, E.J.Villiers, Etienne P. deShah, TushaarBishop, Richard P.Abuya, E.Awino, EliasGachanja, J.Luyai, A.E.Mbwika, F.Muthiani, A.M.Ndegwa, D.M.Njahira, Moses N.Nyanjui, J.K.Onono, F.O.Osaso, J.Saya, R.M.Wildmann, C.Fraser, C.M.Maudlin, I.Gardner, M.J.Morzaria, S.P.Loosmore, S.Gilbert, S.C.Audonnet, J.C.Bruggen, P. van derNene, VishvanathTaracha, E.L.N.[Exploiting host immunity and parasite genomics to develop a robust sub-unit vaccine against East Coast fever in cattle - Where are we?]Exploiting host immunity and parasite genomics to develop a robust sub-unit vaccine against East Coast fever in cattle - Where are we?

Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated by CD8 T cells. Six antigens targeted by CD8 T cells from T. parva-immune cattle of different major histocompatibility complex (MHC) genotypes have been identified, raising the prospect of d...


Graham, S.P.Pelle, RogerYamage, M.Mwangi, D.M.Honda, Y.Mwakubambanya, R.S.Villiers, Etienne P. deAbuya, E.Awino, EliasGachanja, J.Mbwika, F.Muthiani, A.M.Muriuki, C.Nyanjui, J.K.[Characterization of the fine specificity of Bovine CD8 T-Cell responses to defined antigens from the protozoan parasite Theileria parva]Characterization of the fine specificity of Bovine CD8 T-Cell responses to defined antigens from the protozoan parasite Theileria parva

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