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Heartwater, a tick-borne disease of domestic and wild ruminants, is caused by the intracellular rickettsia Ehrlichia ruminantium (previously known as Cowdria ruminantium). It is a major constraint to livestock production throughout subSaharan Africa, and it threatens to invade the Americas, yet t...


Collins, N.E.Liebenberg, J.Villiers, Etienne P. deBrayton, K.A.Louw, E.Pretorius, E.Faber, F.E.Heerden, H. vanJosemans, A.Kleef, M. vanSteyn, H.C.Strijp, M.F. vanZweygarth, E.Jongejan, F.Maillard, J.C.Berthier, D.Botha, M.Joubert, F.Corton, C.H.Thomson, N.R.Allsopp, M.T.Allsopp, B.A.[The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number]The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number

Transcriptome analysis can provide useful data for refining genome sequence annotation. Application of massively parallel signature sequencing (MPSS) revealed reproducible transcription, in multiple MPSS cycles, from 73% of computationally predicted genes in the Theileria parva schizont lifecycle...


Shah, TushaarVilliers, Etienne P. deNene, VishvanathHass, B.Taracha, E.A.Gardner, J.M.Sansom, C.Pelle, RogerBishop, Richard P.[Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)]Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from tic...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-i...


Graham, S.P.Pelle, RogerHonda, Y.Mwangi, D.M.Tonukari, N.J.Yamage, M.Glew, E.J.Villiers, Etienne P. de[Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes]Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes

Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...


Sunter, J.D.Patel, S.P.Skilton, Robert A.Knowles, Donald P.Scoles, Glen A.Nene, VishvanathVilliers, Etienne P. deBishop, Richard P.[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata

Immunity against the bovine protozoan parasite Theileria parva has previously been shown to be mediated through lysis of parasite-infected cells by MHC class I restricted CD8+ cytotoxic T lymphocytes. It is hypothesized that identification of CTL target schizont antigens will aid the development ...


Graham, S.P.Honda, Y.Pelle, RogerMwangi, D.M.Glew, E.J.Villiers, Etienne P. deShah, TushaarBishop, Richard P.Bruggen, P. van derNene, VishvanathTaracha, E.L.N.[A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology]A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology
Bishop, Richard P.Odongo, David O.Mann, D.J.Pearson, T.W.Sugimoto, C.Haines, L.R.Glass, E.Jensen, K.Seitzer, U.Ahmed, J.S.Graham, S.P.Villiers, Etienne P. de[Theileria]Theileria

Haemonchosis is considered to be the most economically important gastrointestinal disease of small ruminants in the tropics and subtropics. However, chemical anthelmintics, which were the mainstay of control, have been compromised by a high prevalence of resistance worldwide. Copper oxide wire pa...


Spickett, A.Villiers, Etienne P. deBoomker, J.Githiori, J.B.Medley, G.F.Stenson, M.O.Waller, P.J.Calitz, F.J.Vatta, A.F.[Tactical treatment with copper oxide wire particles and symptomatic levamisole treatment using the FAMACHA© system in indigenous goats in South Africa]Tactical treatment with copper oxide wire particles and symptomatic levamisole treatment using the FAMACHA© system in indigenous goats in South Africa

We describe the development of TparvaDB, a comprehensive resource to facilitate research towards development of an East Coast fever vaccine, by providing an integrated user-friendly database of all genome and related data currently available for Theileria parva. TparvaDB is based on the Generic M...


Visendi, P.Ng'ang'a, W.Bulimo, WallaceBishop, Richard P.Ochanda, J.Villiers, Etienne P. de[TparvaDB: A database to support Theileria parva vaccine development]TparvaDB: A database to support Theileria parva vaccine development

Camels are the most valuable livestock species in the Horn of Africa and play a pivotal role in the nutritional sustainability for millions of people. Their health status is therefore of utmost importance for the people living in this region. Streptococcus agalactiae, a Group B Streptococcus (GBS...


Fischer, A.Liljander, Anne M.Kaspar, H.Muriuki, C.Fuxelius, H.H.Bongcam-Rudloff, E.Villiers, Etienne P. deHuber, C.A.Frey, J.Daubenberger, C.A.Bishop, Richard P.Younan, M.Jores, Joerg[Camel Streptococcus agalactiae populations are associated with specific disease complexes and acquired the tetracycline resistance gene tetM via a Tn916-like element]Camel Streptococcus agalactiae populations are associated with specific disease complexes and acquired the tetracycline resistance gene tetM via a Tn916-like element

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