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This 9-minute video describes the protocol used in cassava for the analysis pollen tube grow in vivo. Then images of pollen tube growth taken with a fluorescence microscope are presented to illustrate the process. Finally an overall description of the time required for the pollen tube tip to reac...


Pineda, Lizzeth MarcelaRamos, Lisette NataliaWedzony, MariaCeballos, Hernán[Protocol and analysis of in vivo pollen tuve growth = Protocolo y análisis del crecimiento in vivo del tubo polínico en yuca/mandioca]Protocol and analysis of in vivo pollen tuve growth = Protocolo y análisis del crecimiento in vivo del tubo polínico en yuca/mandioca

This 14-minute video describes the protocol used for the collection of female flowers at different ages after anthesis and the development of a protocol of early embryo rescue. The production of doubled haploids through different gynogenesis approaches usually require an early rescue of embryos w...


Buitrago, María EugeniaTabares, EddieLentini, ZaidaVanegas, FreddyWedzony, MariaCeballos, Hernán[Protocol of ovule culture for rescuing embryos in cassava = Protocolo para el cultivo de óvulos en el rescate de embriones en yuca/mandioca]Protocol of ovule culture for rescuing embryos in cassava = Protocolo para el cultivo de óvulos en el rescate de embriones en yuca/mandioca

This 9-minute video describes the protocol used in cassava for the analysis pollen tube grow in vivo. Then images of pollen tube growth taken with a fluorescence microscope are presented to illustrate the process. Finally an overall description of the time required for the pollen tube tip to reac...


Buitrago, María EugeniaLentini, ZaidaTabares, EddieVanegas, FreddyWedzony, MariaCeballos, Hernán[Vibratome technology for histological analysis of embryo development in cassava = Utilización del vibrátomo para análisis histológico del desarrollo embrionario en yuca/mandioca]Vibratome technology for histological analysis of embryo development in cassava = Utilización del vibrátomo para análisis histológico del desarrollo embrionario en yuca/mandioca

This 16-minute video describes the protocol used for the preparation of sections for the histological analysis of cassava pistils of different ages after anthesis. Consolidated results after studying hundreds of samples, present a chronological description of embryo sac and the embryo appearance ...


Ramos, Lisette NataliaWedzony, MariaLentini, ZaidaBuitrago, María EugeniaTabares, EddieVanegas, FreddyCeballos, Hernán[Description of embryo development in cassava = Descripción del desarrollo embrionario en yuca]Description of embryo development in cassava = Descripción del desarrollo embrionario en yuca

This 9-minute video describes the protocol used for the collection of male flowers in cassava for the production of doubled haploids through androgenesis (in vitro microspore or anther culture). Pre-treatments and different culture conditions used in the process to induce cell division in immatur...


Perera, Prasanthi I.P.Golemiec, ElzbietaDedicova, BeataWedzony, MariaHeberle-Bors, ErwinOrdoñez, CarlosCeballos, Hernán[Protocol of anther and microspore culture in cassava (androgenesis) = Protocolo para el cultivo de anteras y microsporas (androgénesis) en yuca/mandioca]Protocol of anther and microspore culture in cassava (androgenesis) = Protocolo para el cultivo de anteras y microsporas (androgénesis) en yuca/mandioca

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