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We previously developed a new simple technique of methylene blue (MB) staining for the discrimination of aluminum (Al)-tolerant protoplasts from 4 plant species (rice, oats, maize and pea). The objectives of the present study were to confirm the applicability of this technique to a wider range of...


Wagatsuma, TadaoKhan, Shahadat HossainRao, Idupulapati M.Wenzl, PeterTawaraya, KeitarouYamamoto, TakanoriKawamura, TakeshiHosogoe, KazumiIshikawa, Satoru[Methylene blue sustainability of root-tip protoplasts as an indicator of aluminum tolerance in a wide range of plant species, cultivars and lines]Methylene blue sustainability of root-tip protoplasts as an indicator of aluminum tolerance in a wide range of plant species, cultivars and lines

Al resistance of signalgrass (Brachiaria decumbens Stapf cv Basilisk), a widely sown tropical forage grass, is outstanding compared with the closely related ruzigrass (Brachiaria ruziziensis Germain and Evrard cv Common) and Al-resistant genotypes of graminaceous crops such as wheat, triticale, a...


Wenzl, PeterPatino, GMChavez, Alba L.Mayer, Jorge EdgardRao, Idupulapati M.[The high level of aluminum resistance in signalgrass is not associated with known mechanism of external aluminum detoxification in root apices]The high level of aluminum resistance in signalgrass is not associated with known mechanism of external aluminum detoxification in root apices

Traditional breeding efforts are expected to be greatly enhanced through collaborative approaches incorporating functional, comparative and structural genomics. Potential benefits of combining genomic tools with traditional breeding have been a source of widespread interest and resulted in numero...


Ishitani, ManabuRao, Idupulapati M.Wenzl, PeterBeebe, Stephen E.Tohme, Joseph M.[Integration of genomics approach with traditional breeding towards improving abiotic stress adaptation: Drought and aluminum toxicity as case studies]Integration of genomics approach with traditional breeding towards improving abiotic stress adaptation: Drought and aluminum toxicity as case studies

Several molecular marker systems have been developed for assessing genetic diversity in crop germplasm collections. A trade-off often exists between the number of loci that can feasibly be sampled by a marker system and the amount of information provided by each locus. We compared the usefulness ...


Hurtado Lopez, PXOlsen, KMBuitrago, C.Ospina, C.Marin, JADuque E., Myriam CristinaVicente, M.C. deWongtiem, PrapitWenzl, PeterKillian, AAdekele, M.Fregene, Martin A.[Comparison of simple sequence repeat (SSR) and diversity array technology (DArT) markers for assessing genetic diversity in cassava Manihot esculenta Crantz)]Comparison of simple sequence repeat (SSR) and diversity array technology (DArT) markers for assessing genetic diversity in cassava Manihot esculenta Crantz)

González Guzman, J.J.Debouck, Daniel G.Wenzl, Peter[Multipurpose leguminous trees for the lowland tropics in CIAT’s genebank.]Multipurpose leguminous trees for the lowland tropics in CIAT’s genebank.

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