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We have previously reported on characterisation of mitochondrial DNA (mtDNA) of Theileria parva (1), an intracellular protozoan parasite of cattle (reviewed in (2). The mtDNA encodes ORFs for apocytochrome b and polypeptides I and III and cytochrome oxidase, contains fragmented rRNA genes and exi...


Shukla, G.C.Nene, Vishvanath[Telomeric features of Theileria parva mitochondrial DNA derived from cycle sequence data of total genomic DNA]Telomeric features of Theileria parva mitochondrial DNA derived from cycle sequence data of total genomic DNA

Bloodstream forms (BSF) and procyclic culture forms (PCF) of African trypanosomes were incubated with a variety of lectins in vitro. Cessation of cell division and profound morphological changes were seen in procyclic forms but not in BSF after incubation with concanvavalin A (Con A). Wheat germ...


Pearson, T.W.Beecroft, R.P.Welburn, S.C.Ruepp, S.Roditi, I.Kuo-Yuan, H.Englund, P.T.Wells, C.W.Murphy, N.B.[The major cell surface glycoprotein procycline is a receptor for induction of a novel form of cell death in African trypanosomes in vitro]The major cell surface glycoprotein procycline is a receptor for induction of a novel form of cell death in African trypanosomes in vitro

As a first step in developing compartment-specific markers for protein trafficking within Theileria parva, we have isolated cDNAs encoding homologues of the small GTP binding proteins Rab1 and Rab4. The T. parva homologue of Rab1 (TpRab1), a protein which regulates vesicular transport between the...


Janoo, R.T.K.Musoke, A.J.Wells, C.Bishop, Richard P.[A Rab1 homologue with a novel isoprenylation signal provides insight into the secretory pathway of Theileria parva]A Rab1 homologue with a novel isoprenylation signal provides insight into the secretory pathway of Theileria parva

When compared with other Apicomplexan organisms. Theileria parva has an execptionally small, 10-12 Mbp. genome. There are only 4 chromosomes, each in the Mbp range, and a complete physical map, based on SfiI linking data, is available for each one. A number of genes and cDNAs have been mapped to ...


Nene, VishvanathMorzaria, S.P.Bishop, Richard P.[Organisation and informational content of the Theileria parva genome]Organisation and informational content of the Theileria parva genome

Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABCI, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing...


Kibe, M.K.Macklin, M.D.Gobright, E.I.Bishop, Richard P.Urakawa, T.Ole-MoiYoi, O.K.[Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva]Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva

During its first two years, the African trypanosome genome project, funded in part by the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), has concentrated on three fundamental areas: (1) gene discovery by single-pass sequencing of cDNAs to produce expre...


Melville, S.E.Majiwa, P.A.O.Donelson, J.E.[Resources available from the African Trypanosome Genome Project]Resources available from the African Trypanosome Genome Project

Ribosomal RNA genes have been cloned from the major species of African trypanosomes. Complete nucleotide sequence composition of the small subunit (SSU) and portions of the large subunit (LSU) ribosomal RNA genes was determined for each of these trypanosome species. In contrast to the situation i...


Urakawa, T.Majiwa, P.A.O.[Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense]Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense
Shapiro, T.A.Showalter, F.[In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles]In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles

Seven cDNA clones corresponding to the rabl, rab2, rab3A, rab3B, rab4, rab5, and rab5 genes were isolated from a human pheochromocytoma cDNA library. They encode 23-25 kDa polyeptides which share =30-50 percent homology and belong to the ras superfamily. The rab1, rab2, rab3A, and rab4 proteins a...


Zahraoui, A.Touchot, N.Chardin, P.Tavitiam, A.[The human rab genes encode a family of GTP - binding proteins related to yeast YPT1 and SEC4 products involved in secretion]The human rab genes encode a family of GTP - binding proteins related to yeast YPT1 and SEC4 products involved in secretion

Cassava is a tropical storage-root crop that serves as a worldwide source of staple food for over 800 million people. Flowering is one of the most important breeding challenges in cassava because in most lines flowering is late and non-synchronized, and flower production is sparse. The FLOWERING ...


Adeyemo, O. SarahChavarriaga Aguirre, PaulTohme, Joseph M.Fregene, Martin A.Davis, Seth J.Setter, Tim L.[Overexpression of Arabidopsis FLOWERING LOCUS T (FT) gene improves floral development in cassava (Manihot esculenta, Crantz)]Overexpression of Arabidopsis FLOWERING LOCUS T (FT) gene improves floral development in cassava (Manihot esculenta, Crantz)

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