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Read, L.K.Fish, W.R.Muthiani, A.M.Stuart, K.[Maxicircle DNA and edited mRNA sequences of closely related trypanosome species: Implications of kRNA editing for evolution at maxicircle]Maxicircle DNA and edited mRNA sequences of closely related trypanosome species: Implications of kRNA editing for evolution at maxicircle
Pfeiffer, S.Pastar, M.Mitter, B.Lippert, K.Hackl, E.Lojan, P.Oswald, A.Sessitsch, A.[Improved group-specific primers based on the full SILVA 16S rRNA gene reference database.]Improved group-specific primers based on the full SILVA 16S rRNA gene reference database.

The genome of cassava common mosaic potexvirus (CsCMV) has been sequenced from cDNA clones and consists of 6376 nucleotides (nt). A 76 nt untranslated region (UTR) at the 5? terminus was followed by ORF1 which potentially encodes a protein of 1449 amino acids (aa). ORFs 2, 3, and 4 were predicted...


Calvert, Lee A.Cuervo Ibáñez, MaritzaOspina, MDFauquet, Claude M.Ramírez, B.C.[Characterization of cassava common mosaic virus and a defective RNA species]Characterization of cassava common mosaic virus and a defective RNA species

Uniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs con...


Abarshi, M.M.Mohammed, I.Jeremiah, S.C.Legg, J.P.Kumar, P.L.Hillocks, R.J.Maruthi, M.N.[Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa]Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa

Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...


Otti, G.Bouvaine, S.Kimata, B.Mkamillo, G.Kumar, P.L.Tomlins, Keith I.Maruthi, M.N.[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

To date, several viruses of different genera have been reported to infect yam (Dioscorea spp.). The full diversity of viruses infecting yam, however, remains to be explored. High-throughput sequencing (HTS) methods are increasingly being used in the discovery of new plant viral genomes. In this s...


Silva, G.Bömer, M.Rathnayake, A.I.Sewe, S.O.Visendi, P.Oyekanmi, J.O.Quain, M.D.Akomeah, B.Kumar, P.L.Seal, S.E.[Molecular characterization of a new virus species identified in yam (Dioscorea spp.) by high-throughput sequencing]Molecular characterization of a new virus species identified in yam (Dioscorea spp.) by high-throughput sequencing

Cuéllar, Wilmer J.Galvez, MarcoFuentes, S.Tugume, Joab K.Kreuze, J.F.[Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)]Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)

The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5’ and 3’ untranslate regions (UTR) and followed by a poly (A) tail. The puta...


Souza, Joao deMuller, G.Pérez, W.Cuéllar, Wilmer J.Kreuze, J.F.[Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru]Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
Beltrán Giraldo, Jorge AlonsoJaimes, HEcheverry, MLadino Franco, YJLópez, D.Duque E., Myriam CristinaChavarriaga Aguirre, PaulTohme, Joseph M.[Quantitative analysis of transgenes in cassava plants using real-time PCR technology]Quantitative analysis of transgenes in cassava plants using real-time PCR technology

Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABCI, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing...


Kibe, M.K.Macklin, M.D.Gobright, E.I.Bishop, Richard P.Urakawa, T.Ole-MoiYoi, O.K.[Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva]Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva

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