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Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...


Otti, G.Bouvaine, S.Kimata, B.Mkamillo, G.Kumar, P.L.Tomlins, Keith I.Maruthi, M.N.[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants
Tegally, A.Jaufeerally-Fakim, Y.Dulloo, M.E.[Molecular characterisation of Solanum melongena L. and the crop wild relatives, S. violaceum Ortega and S. torvum Sw., using phylogenetic/DNA barcoding markers]Molecular characterisation of Solanum melongena L. and the crop wild relatives, S. violaceum Ortega and S. torvum Sw., using phylogenetic/DNA barcoding markers

Cucumber mosaic virus (CMV) occurs worldwide and is a harmful pathogen for pepper (Capsicum spp.). Transmission of the virus is normally by insect. The possibility of transmission by seed is less clear. Accessions collected from eight states in Nigeria were evaluated to determine seed transmissib...


Arogundade, O.Balogun, O.S.Kumar, P.L.[Seed transmissibility of Cucumber mosaic virus in Capsicum species]Seed transmissibility of Cucumber mosaic virus in Capsicum species

Prentice, K.Pertry, I.Christiaens, O.Bauters, L.Bailey, M.Niblett, C.Ghislain, M.Gheysen, G.Smagghe, G.[Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae]Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae

Germplasm of the calabash tree (Crescentia cujete L.) was collected in five major regions of Colombia, i.e. the Andes, Caribbean, Amazon, Orinoco, and Pacific regions. Collecting this multipurpose tree was guided by the indigenous knowledge of farmers and artisans in each region. Large variation ...


Arango Ulloa, AJBohórquez Cháux, AdrianaDuque E., Myriam CristinaMaass, Brigitte L.[Diversity of the calabash tree (Crescentia cujete L.) in Colombia]Diversity of the calabash tree (Crescentia cujete L.) in Colombia

Phaseolus beans are distributed worldwide and are cultivated in the tropics, subtropics, and temperate zones. The common bean is the most important grain legume for direct human consumption in the world. The objectives of this study were to isolate microsatellite repeats and to establish their di...


Gaitán Solís, ElianaDuque E., Myriam CristinaEdwards, K.J.Tohme, Joseph M.[Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp]Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp

Coevolution of the angular leaf spot pathogen, Phaeoisariopsis griseola, with its common bean host has been demonstrated, and P. griseola isolates have been divided into Andean and Mesoamerican groups that correspond to defined bean gene pools. Recent characterization of P. griseola isolates from...


Mahuku, George S.Henriquez Naranjo, MAMuñoz, J.Buruchara, Robin A.[Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola]Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola
Beltrán Giraldo, Jorge AlonsoJaimes, HEcheverry, MLadino Franco, YJLópez, D.Duque E., Myriam CristinaChavarriaga Aguirre, PaulTohme, Joseph M.[Quantitative analysis of transgenes in cassava plants using real-time PCR technology]Quantitative analysis of transgenes in cassava plants using real-time PCR technology

Low soil P availability is a primary constraint to common bean (Phaseolus vulgaris L.) production in Latin America and Africa. Substantial genotypic variation in bean adaptation to low phosphorus (LP) availability has been linked with root traits that enhance the efficiency of soil foraging. The ...


Beebe, Stephen E.Rojas, MYan, XBlair, Matthew W.Pedraza García, FabioMuñoz Arboleda, FernandoTohme, Joseph M.Lynch, Jonathan P.[Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean]Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean

Tithonia diversifolia (Mexican sunflower) is a shrub commonly used as a green manure crop in Central and South America, Asia and Africa as it accumulates high levels of phosphorus and other nutrients, even in depleted soils. In root samples collected from the global distribution of Tithonia, we e...


Sharrock, R.A.Sinclair, Fergus L.Gliddon, C.Rao, Idupulapati M.Barrios, EdmundoMustonen, P.J.Smithson, P.Jones, D.L.Godbold, D.L.[A global assessment using PCR techniques of mycorrhizal fungal populations colonising Tithonia diversifolia]A global assessment using PCR techniques of mycorrhizal fungal populations colonising Tithonia diversifolia

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