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We applied the cDNA-AFLP (amplified fragment length polymorphism) technique to mRNA from the parents of a cassava (Manihot esculenta) genetic mapping population, and obtained more than 500 transcript-derived fragments (TDFs) that were unique in either parent. A subset of 50 TDFs were cloned and s...


Suarez, MCBernal, A.Gutiérrez, J.Tohme, Joseph M.Fregene, Martin A.[Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)]Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)

Cassava mosaic disease (CMD) is the most-important disease of cassava (Manihot esculenta) in Africa, and is a potential threat to Latin American (LA) cassava production. Although this viral disease is still unknown in LA, its vector – the whitefly – has recently been found. The disease is best co...


Akano, A.O.Dixon, Alfred G.O.Mba, CBarrera Sabogal, EFregene, Martin A.[Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease]Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease

Bean golden yellow mosaic virus (BGYMV) is a whitefly-transmitted geminivirus of the Begomovirus family that causes important yield losses to common beans grown in tropical and sub-tropical countries of Latin America and the Caribbean. A major resistance gene that has been widely deployed in this...


Blair, Matthew W.Rodríguez, L.M.Pedraza García, FabioMorales, Francisco JoséBeebe, Stephen E.[Genetic mapping of the bean golden yellow mosaic geminivirus resistance gene bgm-1 and linkage with potyvirus resistance in common bean (Phaseolus vulgaris L.)]Genetic mapping of the bean golden yellow mosaic geminivirus resistance gene bgm-1 and linkage with potyvirus resistance in common bean (Phaseolus vulgaris L.)

A genetic map was constructed with specific PCRs, DALPs and AFLPs using F8-generation sunflower recombinant inbred lines. RI lines generated from a F2 population of one cross between the two cultivated inbred lines HA89 (maintainer for Pet1 CMS) and LR4 (restorer for Pet1 CMS) were used. A total ...


Langar, KLorieux, MDesmarais, EGriveau, YGentzbittel, IBerville, A[Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines]Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines

Microsatellites or simple sequence repeats (SSR) are the markers of choice for molecular genetic mapping and marker-assisted selection in many crop species. A microsatellite-based linkage map of cassava was drawn using SSR markers and a F2 population consisting of 268 individuals. The F2 populati...


Okogbenin, EmmanuelMarin, JAFregene, Martin A.[An SSR-based molecular genetic map of cassava]An SSR-based molecular genetic map of cassava

A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to...


Blair, Matthew W.Pedraza García, FabioBuendía Castellanos, Hector F.Gaitán Solís, ElianaBeebe, Stephen E.Gepts, Paul L.Tohme, Joseph M.[Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)]Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)

International Laboratory for Research on Animal Diseases[ILRAD Reports. Volume 7. Number 4. October 1989]ILRAD Reports. Volume 7. Number 4. October 1989

Sharma, S.K.Bolser, D.Boer, J. de.Sonderkaer, M.Amoros, W.Carboni, M.F.D'Ambrosio, J.M.Cruz, G. de laGenova, A. diDouches, D.S.Eguiluz, M.Guo, X.Guzmán, F.Hackett, C.A.Hamilton, J.P.Li, G.Li, Y.Lozano, R.Maass, A.Marshall, D.Martínez, D.McLean, K.Mejia, N.Milne, L.Munive, S.Nagy, I.Ponce, O.Ramírez, M.Simon, R.Thomson, S.J.Torres, Y.Waugh, R.Zhang, Z.Huang, S.Visser, Richard G.F.Bachem, C.W.B.Sagredo, B.Feingold, S.E.Orjeda, G.Veilleux, R.E.Bonierbale, Merideth W.Jacobs, J.M.E.Milbourne, D.Martin, D.M.A.Bryan, G.J.[Construction of reference chromosome-scale pseudomolecules for potato: Integrating the potato genome with genetic and physical maps.]Construction of reference chromosome-scale pseudomolecules for potato: Integrating the potato genome with genetic and physical maps.
Akinbo, OlalekanFregene, Martin A.Labuschagne, M.T.[Quantitative trait loci (QTL) mapping of protein content in backcross derivatives of inter-specific hybrids from M. esculenta sub spp. Flabellifolia and cassava (Manihot esculenta Crantz)]Quantitative trait loci (QTL) mapping of protein content in backcross derivatives of inter-specific hybrids from M. esculenta sub spp. Flabellifolia and cassava (Manihot esculenta Crantz)

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