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Pelle, RogerMurphy, N.B.[In vivo UV cross-linking hybridization, a powerful technique for isolating RNA binding proteins: Application to trypanosome mini-exon derived RNA]In vivo UV cross-linking hybridization, a powerful technique for isolating RNA binding proteins: Application to trypanosome mini-exon derived RNA

The cDNA coding for the soluble form of bovine stem cell factor (boSCF-Ala-105) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. ...


Mertens, B.Muriuki, C.Muiya, P.Andrianarivo, A.Mwangi, S.Logan-Henfrey, L.L.[Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense]Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense

The Potyviridae is the largest family of RNA plant viruses, members of which have single-stranded, positive-sense RNA genomes and flexuous filamentous particles 680–900 nm long and 11–20 nm wide. There are eight genera, distinguished by the host range, genomic features and phylogeny of the member...


Wylie, S.J.Adams, M.Chalam, C.Kreuze, J.F.López Moya, J.J.Ohshima, K.Praveen, S.Rabenstein, F.Stenger, D.Wang, A.Zerbini, F.M.[ICTV virus taxonomy profile: Potyviridae.]ICTV virus taxonomy profile: Potyviridae.
Nolt, Barry L.Pineda López, BenjamínVelasco, Ana Cecilia[Surveys of cassava plantations in Colombia for virus and virus- like diseases]Surveys of cassava plantations in Colombia for virus and virus- like diseases
Arango, JacoboBeltrán, Jesús A.Nuñez, JonathanChavarriaga Aguirre, Paul[Evidence of epigenetic mechanisms affecting carotenoids]Evidence of epigenetic mechanisms affecting carotenoids

Small interfering RNA (siRNA) duplexes are short (usually 21 to 24 bp) double-stranded RNAs (dsRNAs) with several overhanging nucleotides at both 5'- and 3'-ends. It has been found that siRNA duplexes bind the RNA-induced silencing complex (RISC) and cleave the sense strands with endonucleases. I...


Niu, X.Sun, Y.Chen, Z.Li, R.Padmanabhan, C.Ruan, J.Kreuze, J.F.Ling, K.S.Fei, Z.J.Gao, S.[Using small RNA-seq data to detect siRNA duplexes induced by plant viruses.]Using small RNA-seq data to detect siRNA duplexes induced by plant viruses.

We have evaluated whether sequence polymorphisms in the rRNA intergenic spacer region can be used to study the relatedness c.' two subspecies of Trypanosoma brucei. Thirteen T. bnrcei isolates made up of 6 T. b. brucei and 7 T. b. gambiense were analyzed using restriction fragment length polymorp...


Agbo, E.C.Majiwa, P.A.O.Classsen, E.J.H.M.Roos, M.H.[Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization]Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization

RNA interference (RNAi) is a natural defense mechanism triggered by double-stranded RNA (dsRNA) for protection of cells against foreign parasitic nucleic acids. The RNase III enzyme Dicer processes dsRNAs into short small interfering RNAs (siRNAs) that degrade the specific mRNA. RNAi has been dem...


Ocimati, W.Kiggundu, A.Bailey, A.Niblett, C.L.Pedun, H.Tazuba, A.F.Tushemereirwe, W.K.Karamura, E.B.[Suppression of the ubiquitin E2 gene through RNA interference causes mortality in the banana weevil, Cosmopolites sordidus (Germar)]Suppression of the ubiquitin E2 gene through RNA interference causes mortality in the banana weevil, Cosmopolites sordidus (Germar)

Storage roots of cassava (Manihot esculenta Crantz) exhibit a rapid post-harvest physiological deterioration (PPD) response that can occur within 24 72 h of harvest. PPD is an enzymatically mediated oxidative process with parallels to plant wound, senescence and defence responses. To characterise...


Reilly, KBernal Franco, D.M.Cortés Bermúdez, D.F.Gómez Vásquez, RTohme, Joseph M.Beeching, JR[Towards identifying the full set of genes expressed during cassava post-harvest physiological deterioration]Towards identifying the full set of genes expressed during cassava post-harvest physiological deterioration

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