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Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...


Sánchez, I.Angel, F.Grum, M.Duque E., Myriam CristinaLobo Arias, MTohme, Joseph M.Roca, William M.[Variability of chloroplast DNA in the genus Passiflora L.]Variability of chloroplast DNA in the genus Passiflora L.

Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...


Ramírez, H.Mayer, Jorge EdgardRoca, William M.Tohme, Joseph M.López, Y.[Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético]Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético

Background: Gene-based (genic) microsatellites are a useful tool for plant genetics and simple sequence repeat loci can often be found in coding regions of the genome. While EST sequencing can be used to discover genic microsatellites, direct screening of cDNA libraries for repeat motifs can save...


Blair, Matthew W.Muñoz Torres, M.C.Giraldo, M.C.Pedraza, F[Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.)]Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.)

Bean dwarf mosaic geminivirus (BDMV) was shown to have a bipartite genome (DNA-A and DNA-B) from restriction map and sequence data comparisons of cloned DNAs. The nucleotide sequences of the infectious clones of DNA-A and DNA-B were determined. Computer analyses showed that the bipartite genome o...


Hidayat, SHGilbertson, R.L.Hanson, SFMorales, Francisco JoséAhlquist, P.G.Russell, DRMaxwell, D.P.[Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus]Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus

Cassava vein mosaic virus (CVMV) was found to be widespread throughout the north-eastern region of Brazil. The complete sequence of CVMV was determined, and the genome was 8158 bp in size. A cytosolic initiator methionine tRNA (tRNAmeti)-binding site that probably acts as a primer for minus-stran...


Calvert, Lee A.Ospina, MDShepherd, RJ[Characterization of cassava vein mosaic virus: A distinct plant pararetrovirus]Characterization of cassava vein mosaic virus: A distinct plant pararetrovirus

La Antracnosis, causada por el hongo Colletotrichum gloeosporioides, ocasiona pérdidas hasta del 90% en huertos no tecnificados de guanábana. Para el control de la enfermedad se requiere conocer bien la sintomatología y las características del agente causal. Teniendo en cuenta que existe poca inf...


Álvarez, E.Ospina, CAMejía De Los Rios, J.F.Llano Rodríguez, Germán Alberto[Caracterización morfológica, patogénica y genética del agente causal de la antracnosis (Colletotrichum gloeosporioides) en guanábana (Annona muricata) en el Valle del Cauca]Caracterización morfológica, patogénica y genética del agente causal de la antracnosis (Colletotrichum gloeosporioides) en guanábana (Annona muricata) en el Valle del Cauca
Utsumi, YTanaka, MMorosawa, TKurotani, AYoshida, TMochida, KMatsui, AUmemura, YIshitani, ManabuShinozaki, KSakurai, TSeki, M[Transcriptome analysis using a high-density oligomicroarray under drought stress in various genotypes of cassava: an important tropical crop]Transcriptome analysis using a high-density oligomicroarray under drought stress in various genotypes of cassava: an important tropical crop
Blair, Matthew W.Brondani, Rosana Pereira V.Díaz, Lucy M.Peloso, Maria José del[Diversity and population structure of common bean from Brazil]Diversity and population structure of common bean from Brazil

Background Cassava, Manihot esculenta Crantz, is one of the most important crops world-wide representing the staple security for more than one billion of people. The development of dense genetic and physical maps, as the basis for implementing genetic and molecular approaches to accelerate the ra...


Soto, Johana CarolinaOrtiz, Juan FelipePerlaza-Jiménez, LauraVásquez, Andrea XimenaBecerra López Lavelle, Luis AugustoMathew, BobyLéon, JensBernal, Adriana JimenaBallvora, AgimLópez Carrascal, Camilo Ernesto[A genetic map of cassava (Manihot esculenta Crantz) with integrated physical mapping of immunity-related genes]A genetic map of cassava (Manihot esculenta Crantz) with integrated physical mapping of immunity-related genes

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