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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

CIAT activities in developing biotechnology applications to cassava in the last decade are described. The strategies to identify research topics and implement in house capabilities and an international cooperative effort in cassava biotechnology are emphasized. Successful integration of selected ...


Roca, William M.Henry, GuyAngel, F.Sarria, R[Biotechnology research applied to cassava improvement at the International Center of Tropical Agriculture (CIAT)]Biotechnology research applied to cassava improvement at the International Center of Tropical Agriculture (CIAT)

Cassava (Manihot esculenta Crantz) germplasm collections are conventionally maintained by continuous vegetative propagation in the field. Tissue culture techniques provide a more convenient way to conserve germplasm. The cassava in vitro gene bank held in trust at CIAT comprises nearly 6000 acces...


Angel, F.Barney, Victoria E.Tohme, Joseph M.Roca, William M.[Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage]Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage

Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledon...


Sarria, RATorres Castro, Edgar AlonsoAngel, F.Chavarriaga Aguirre, PaulRoca, William M.[Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation]Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation

Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...


Fregene, Martin A.Okogbenin, EmmanuelMba, CAngel, F.Suarez, MCGutiérrez A., Janeth PatriciaChavarriaga Aguirre, PaulRoca, William M.Bonierbale, Merideth W.Tohme, Joseph M.[Genome mapping in cassava improvement: Challenges, achievements and opportunities]Genome mapping in cassava improvement: Challenges, achievements and opportunities

A preliminary linkage map of cassava (Manihot esculenta Crantz) to increase the efficiency of introgression of specific chromosomes or chromosome fragments from exotic cassava germplasm into various genetic backgrounds and localization of genes of interest in cassava improvement schemes has been ...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Maya, M.M.Bonierbale, Merideth W.Tohme, Joseph M.Iglesias, C.A.[A linkage map of cassava (Manihot esculenta Crantz) based on RFLP and RAPD markers]A linkage map of cassava (Manihot esculenta Crantz) based on RFLP and RAPD markers

In large ex situ germplasm collections, such as that maintained at CIAT for cassava, it is common to observe duplicates. The presence of duplicate clones increases the cost of conservation without contributing to representation or the available genetic base for crop improvement. To identify group...


Ocampo Nahar, César H.Angel, F.Jiménez, A.Jaramillo O, GHershey, Clair H.Granados F, EIglesias, C.A.[DNA fingerprinting to confirm possible genetic duplicates in cassava germplasm]DNA fingerprinting to confirm possible genetic duplicates in cassava germplasm

Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Se...


Gómez, R.Angel, F.Bonierbale, Merideth W.Rodríguez, F.Tohme, Joseph M.Roca, William M.[Selecting heterozygous parents and single-dose markers for genetic mapping in cassava]Selecting heterozygous parents and single-dose markers for genetic mapping in cassava

The construction of a detailed genetic map of cassava (Manihot esculenta Crantz) will be useful to tag agronomically important trais as well as to isolate and to clone cassava genes in the future. This map will also contribute to the understanding of cassava genomic structure and evolution. As a ...


Angel, F.Giraldo, F.Gómez, R.Iglesias, C.A.Tohme, Joseph M.Roca, William M.[Use of RFLPs and RAPDs in cassava genomic studies]Use of RFLPs and RAPDs in cassava genomic studies

Angel, F.Arias Garzón, D.I.Roca, William M.[DNA polymorphism and DNA fingerprinting of cassava : final report collaborative project CIAT-IBPGR]DNA polymorphism and DNA fingerprinting of cassava : final report collaborative project CIAT-IBPGR

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