A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...
Fregene, Martin A.
,
Angel, F.
,
Gómez, R.
,
Rodríguez, F.
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
Tohme, Joseph M.
,
Bonierbale, Merideth W.
,
[A molecular genetic map of cassava (Manihot esculenta Crantz)]
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A molecular genetic map of cassava (Manihot esculenta Crantz)
Chloroplast DNA (cp) and nuclear ribosomal DNA (rDNA) variation was investigated in 45 accessions of cultivated and wild Manihot species. Ten independent mutations, 8 point mutations and 2 length mutations were identified, using eight restriction enzymes and 12 heterologous cpDNA probes from mung...
Fregene, Martin A.
,
Vargas, J.
,
Ikea, J.
,
Angel, F.
,
Thome, J.
,
Asiedu, Robert
,
Akoroda, M.O.
,
Roca, W.
,
[Variability of chloroplast DNA and nuclear ribosomal DNA in cassava (Manihot esculenta Crantz) and its wild relatives]
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Variability of chloroplast DNA and nuclear ribosomal DNA in cassava (Manihot esculenta Crantz) and its wild relatives
CIAT activities in developing biotechnology applications to cassava in the last decade are described. The strategies to identify research topics and implement in house capabilities and an international cooperative effort in cassava biotechnology are emphasized. Successful integration of selected ...
Roca, William M.
,
Henry, Guy
,
Angel, F.
,
Sarria, R
,
[Biotechnology research applied to cassava improvement at the International Center of Tropical Agriculture (CIAT)]
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Biotechnology research applied to cassava improvement at the International Center of Tropical Agriculture (CIAT)
Cassava (Manihot esculenta Crantz) germplasm collections are conventionally maintained by continuous vegetative propagation in the field. Tissue culture techniques provide a more convenient way to conserve germplasm. The cassava in vitro gene bank held in trust at CIAT comprises nearly 6000 acces...
Angel, F.
,
Barney, Victoria E.
,
Tohme, Joseph M.
,
Roca, William M.
,
[Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage]
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Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage
Roca, William M.
,
Mafla, G.
,
Escobar, R.
,
Angel, F.
,
[Tissue culture methods for germplasm conservation]
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Tissue culture methods for germplasm conservation
Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...
Sánchez, I.
,
Angel, F.
,
Grum, M.
,
Duque E., Myriam Cristina
,
Lobo Arias, M
,
Tohme, Joseph M.
,
Roca, William M.
,
[Variability of chloroplast DNA in the genus Passiflora L.]
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Variability of chloroplast DNA in the genus Passiflora L.
EI síndrome de la hoja amarilla es una enfermedad de la calla de azúcar que fue detectada en Colombia por primera vez en 1998. Cenicaña está tratando de lograr un control eficiente de la enfermedad utilizando diferentes herramientas pertenecientes a diversas áreas como entomología, patología, bio...
Rangel, MP
,
Tabares, E
,
Lentini, Z
,
Tohme, Joseph M.
,
Mirkov, E
,
Victoria Kafure, JI
,
Angel, F.
,
[Transformación de plantas de caña de azúcar susceptibles al síndrome de la hoja amarilla = Transformation of sugar cane plants susceptible to yellow leaf virus]
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Transformación de plantas de caña de azúcar susceptibles al síndrome de la hoja amarilla = Transformation of sugar cane plants susceptible to yellow leaf virus
Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledon...
Sarria, RA
,
Torres Castro, Edgar Alonso
,
Angel, F.
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
[Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation]
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Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation
Genetic analysis based on Random Amplified Polymorphic DNA (RAPD) was carried out on 52 accessions representing 14 species of the genus Passiflora L. using 50 random 10-mer primers. A dendrogram constructed using the Dice similarity coefficient and the UPGMA algorithm based on 626 reproducible po...
Fajardo, Diego
,
Angel, F.
,
Grum, M.
,
Tohme, Joseph M.
,
Lobo Arias, M
,
Roca, William M.
,
Sánchez, I.
,
[Genetic variation analysis of the genus Passiflora L. using RAPD markers]
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Genetic variation analysis of the genus Passiflora L. using RAPD markers
Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...
Fregene, Martin A.
,
Okogbenin, Emmanuel
,
Mba, C
,
Angel, F.
,
Suarez, MC
,
Gutiérrez A., Janeth Patricia
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
Bonierbale, Merideth W.
,
Tohme, Joseph M.
,
[Genome mapping in cassava improvement: Challenges, achievements and opportunities]
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Genome mapping in cassava improvement: Challenges, achievements and opportunities