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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

Cassava (Manihot esculenta Crantz) germplasm collections are conventionally maintained by continuous vegetative propagation in the field. Tissue culture techniques provide a more convenient way to conserve germplasm. The cassava in vitro gene bank held in trust at CIAT comprises nearly 6000 acces...


Angel, F.Barney, Victoria E.Tohme, Joseph M.Roca, William M.[Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage]Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage

Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...


Sánchez, I.Angel, F.Grum, M.Duque E., Myriam CristinaLobo Arias, MTohme, Joseph M.Roca, William M.[Variability of chloroplast DNA in the genus Passiflora L.]Variability of chloroplast DNA in the genus Passiflora L.

EI síndrome de la hoja amarilla es una enfermedad de la calla de azúcar que fue detectada en Colombia por primera vez en 1998. Cenicaña está tratando de lograr un control eficiente de la enfermedad utilizando diferentes herramientas pertenecientes a diversas áreas como entomología, patología, bio...


Rangel, MPTabares, ELentini, ZTohme, Joseph M.Mirkov, EVictoria Kafure, JIAngel, F.[Transformación de plantas de caña de azúcar susceptibles al síndrome de la hoja amarilla = Transformation of sugar cane plants susceptible to yellow leaf virus]Transformación de plantas de caña de azúcar susceptibles al síndrome de la hoja amarilla = Transformation of sugar cane plants susceptible to yellow leaf virus

Genetic analysis based on Random Amplified Polymorphic DNA (RAPD) was carried out on 52 accessions representing 14 species of the genus Passiflora L. using 50 random 10-mer primers. A dendrogram constructed using the Dice similarity coefficient and the UPGMA algorithm based on 626 reproducible po...


Fajardo, DiegoAngel, F.Grum, M.Tohme, Joseph M.Lobo Arias, MRoca, William M.Sánchez, I.[Genetic variation analysis of the genus Passiflora L. using RAPD markers]Genetic variation analysis of the genus Passiflora L. using RAPD markers

Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...


Fregene, Martin A.Okogbenin, EmmanuelMba, CAngel, F.Suarez, MCGutiérrez A., Janeth PatriciaChavarriaga Aguirre, PaulRoca, William M.Bonierbale, Merideth W.Tohme, Joseph M.[Genome mapping in cassava improvement: Challenges, achievements and opportunities]Genome mapping in cassava improvement: Challenges, achievements and opportunities
Sánchez, I.Angel, F.Fajardo, DiegoCastillo, MFLobo Arias, MGrum, M.Tohme, Joseph M.Roca, William M.[Caracterización por medio de marcadores moleculares como base en el mejoramiento genético del género Passiflora L.]Caracterización por medio de marcadores moleculares como base en el mejoramiento genético del género Passiflora L.

A preliminary linkage map of cassava (Manihot esculenta Crantz) to increase the efficiency of introgression of specific chromosomes or chromosome fragments from exotic cassava germplasm into various genetic backgrounds and localization of genes of interest in cassava improvement schemes has been ...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Maya, M.M.Bonierbale, Merideth W.Tohme, Joseph M.Iglesias, C.A.[A linkage map of cassava (Manihot esculenta Crantz) based on RFLP and RAPD markers]A linkage map of cassava (Manihot esculenta Crantz) based on RFLP and RAPD markers

Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Se...


Gómez, R.Angel, F.Bonierbale, Merideth W.Rodríguez, F.Tohme, Joseph M.Roca, William M.[Selecting heterozygous parents and single-dose markers for genetic mapping in cassava]Selecting heterozygous parents and single-dose markers for genetic mapping in cassava

The construction of a detailed genetic map of cassava (Manihot esculenta Crantz) will be useful to tag agronomically important trais as well as to isolate and to clone cassava genes in the future. This map will also contribute to the understanding of cassava genomic structure and evolution. As a ...


Angel, F.Giraldo, F.Gómez, R.Iglesias, C.A.Tohme, Joseph M.Roca, William M.[Use of RFLPs and RAPDs in cassava genomic studies]Use of RFLPs and RAPDs in cassava genomic studies

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