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Roca, William M.Mafla, G.Escobar, R.Angel, F.[Tissue culture methods for germplasm conservation]Tissue culture methods for germplasm conservation

A preliminary linkage map of cassava (Manihot esculenta Crantz) to increase the efficiency of introgression of specific chromosomes or chromosome fragments from exotic cassava germplasm into various genetic backgrounds and localization of genes of interest in cassava improvement schemes has been ...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Maya, M.M.Bonierbale, Merideth W.Tohme, Joseph M.Iglesias, C.A.[A linkage map of cassava (Manihot esculenta Crantz) based on RFLP and RAPD markers]A linkage map of cassava (Manihot esculenta Crantz) based on RFLP and RAPD markers

In large ex situ germplasm collections, such as that maintained at CIAT for cassava, it is common to observe duplicates. The presence of duplicate clones increases the cost of conservation without contributing to representation or the available genetic base for crop improvement. To identify group...


Ocampo Nahar, César H.Angel, F.Jiménez, A.Jaramillo O, GHershey, Clair H.Granados F, EIglesias, C.A.[DNA fingerprinting to confirm possible genetic duplicates in cassava germplasm]DNA fingerprinting to confirm possible genetic duplicates in cassava germplasm

Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Se...


Gómez, R.Angel, F.Bonierbale, Merideth W.Rodríguez, F.Tohme, Joseph M.Roca, William M.[Selecting heterozygous parents and single-dose markers for genetic mapping in cassava]Selecting heterozygous parents and single-dose markers for genetic mapping in cassava

The construction of a detailed genetic map of cassava (Manihot esculenta Crantz) will be useful to tag agronomically important trais as well as to isolate and to clone cassava genes in the future. This map will also contribute to the understanding of cassava genomic structure and evolution. As a ...


Angel, F.Giraldo, F.Gómez, R.Iglesias, C.A.Tohme, Joseph M.Roca, William M.[Use of RFLPs and RAPDs in cassava genomic studies]Use of RFLPs and RAPDs in cassava genomic studies

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