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Arias, Marisa[Current status of serological and nucleic acid based diagnostic techniques for ASF]Current status of serological and nucleic acid based diagnostic techniques for ASF

Complete sequencing of p54-gene from 67 European, American, and West and East African Swine Fever virus (ASFV) isolates revealed that West African and European ASFV isolates classified within the predominant Genotype I according to partial sequencing of p72 were discriminated into four major sub-...


Gallardo, C.Mwaengo, D.M.Macharia, J.M.Arias, MarisaTaracha, E.A.Soler Garzón, AlvaroOkoth, S.Martin, E.Kasiti, J.Bishop, Richard P.[Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes]Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes
Okoth, Edward A.Macharia, J.M.Omore, Amos O.Pelayo, V.Bulimo, WallaceArias, MarisaKitala, P.Lekolol, I.Mijele, D.Bishop, Richard P.[An African swine fever virus genotype present in both the resistant bushpig (Potamochoerus larvatus) and adjacent domestic pig populations does not induce detectable clinical ASF symptoms]An African swine fever virus genotype present in both the resistant bushpig (Potamochoerus larvatus) and adjacent domestic pig populations does not induce detectable clinical ASF symptoms

Viral molecular epidemiology has traditionally analyzed variation in single genes. Whole genome phylogenetic analysis of 123 concatenated genes from 11 ASFV genomes, including E75, a newly sequenced virulent isolate from Spain, identified two clusters. One contained South African isolates from ti...


Villiers, Etienne P. deGallardo, C.Arias, MarisaSilva, M. daUpton, C.Martin, R.Bishop, Richard P.[Phylogenomic analysis of 11 complete African swine fever virus genome sequences]Phylogenomic analysis of 11 complete African swine fever virus genome sequences

The role of the ancestral sylvatic cycle of the African swine fever virus (ASFV) is not well understood in the endemic areas of eastern Africa. We therefore analysed the ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Ornithodorus porcinus ...


Gallardo, C.Okoth, S.Pelayo, V.Anchuelo, R.Martin, E.Simon, A.Llorente, A.Nieto, R.Soler Garzón, AlvaroMartin, R.Arias, MarisaBishop, Richard P.[African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site]African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site

African swine fever virus p72 genotype IX, associated with outbreaks in eastern Africa, is cocirculating in the Republic of the Congo with West African genotype I. Data suggest that viruses from eastern Africa are moving into western Africa, increasing the threat of outbreaks caused by novel viru...


Gallardo, C.Anchuelo, R.Pelayo, V.Poudevigne, F.León, T.Nzoussi, J.Bishop, Richard P.Pérez, C.Soler Garzón, AlvaroNieto, R.Martín, H.Arias, Marisa[African Swine Fever Virus p72 Genotype IX in Domestic Pigs, Congo, 2009]African Swine Fever Virus p72 Genotype IX in Domestic Pigs, Congo, 2009

A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous control, has been developed and validated by four National Reference Laboratories of the European Union for Af...


Tignon, M.Gallardo, C.Iscaro, C.Hutet, E.Stede, Y. van derKolvasov, D.Mia, G.M. dePotier, M.F. leBishop, Richard P.Arias, MarisaKoenen, F.[Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus]Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus

We describe a horizontal survey of African swine fever virus (ASFV) prevalence and risk factors associated with virus infection in domestic pigs in two contrasting production systems in Kenya. A free range/tethering, low input production system in Ndhiwa District of South-western Kenya is compare...


Okoth, Edward A.Gallardo, C.Macharia, J.M.Omore, Amos O.Pelayo, V.Bulimo, D.W.Arias, MarisaKitala, P.Baboon, K.Lekolol, I.Mijele, D.Bishop, Richard P.[Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya]Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya

A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the...


Fernández Pinero, J.Gallardo, C.Elizalde, M.Robles, A.Gómez, C.Bishop, Richard P.Heath, L.Couacy-Hyman, E.Fasina, F.O.Pelayo, V.Soler Garzón, AlvaroArias, Marisa[Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library]Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library

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