For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...
Anthracnose, caused by Colletotrichum gloeosporioides Penz., is the most severe foliar disease of water yam (Dioscorea alata) worldwide. Population genetic analyses can yield useful insights into the evolutionary potential of C. gloeosporioides and thus lead to the development of appropriate dise...
Abang, Mathew M.
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Asiedu, Robert
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Hoffmann, P.
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Wolf, G.A.
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Mignouna, H.D.
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Winter, S.
,
[Pathogenic and genetic variability among Colletotrichum gloeosporioides isolates from different yam hosts in the agroecological zones in Nigeria]
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Pathogenic and genetic variability among Colletotrichum gloeosporioides isolates from different yam hosts in the agroecological zones in Nigeria
Colletotrichum gloeosporioides causes anthracnose, the most severe foliar disease of field-grown water yam (Dioscorea alata). The inheritance of resistance to a moderately virulent (FGS) strain of the pathogen was investigated in crosses between tetraploid D. alata genotypes: TDa 95/00328 (resist...
Mignouna, H.D.
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Abang, Mathew M.
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Green, K.R.
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Asiedu, Robert
,
[Inheritance of resistance in water yam (Disocorea alata) to anthracnose (Colletotrichum gloeosporioides)]
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Inheritance of resistance in water yam (Disocorea alata) to anthracnose (Colletotrichum gloeosporioides)
Highly virulent, slow-growing grey (SGG); moderately virulent, fast-growing salmon (FGS); and avirulent/weakly virulent, fast-growing grey (FGG) forms of Colletotrichum gloeosporioides have been described from yam (Dioscorea spp.), but little is known about their chemodiversity or the role of tox...
Abang, Mathew M.
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Abraham, W.R.
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Asiedu, Robert
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Hoffmann, P.
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Wolf, G.
,
Winter, S.
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[Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.)]
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Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.)
Background: Anthracnose (Colletotrichum gloeosporioides) is a major limiting factor in the production of yam (Dioscorea spp.) worldwide. Availability of high quality sequence information is necessary for designing molecular markers associated with resistance. However, very limited sequence inform...
Narina, S.S.
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Buyyarapu, R.
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Kottapalli, K.R.
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Sartie, A.M.
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Ali, M.I.
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Asiedu, Robert
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Hodeba, M.
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Sayre, B.L.
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Scheffler, B.E.
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[Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)]
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Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)
Water yam (Dioscorea alata L.) is the most widely cultivated yam species globally. The major limitation to the profitable and sustainable production of D. alata is its susceptibility to anthracnose disease. The availability of resistant varieties could potentially form the cornerstone of an integ...
Abang, Mathew M.
,
Winter, S.
,
Mignouna, H.D.
,
Green, K.R.
,
Asiedu, Robert
,
[Molecular taxonomic, epidemiological and population genetic approaches to understanding yam anthracnose disease]
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Molecular taxonomic, epidemiological and population genetic approaches to understanding yam anthracnose disease
Yam anthracnose and virus diseases are the most important biotic constraints affecting yam production in the world. Six Dioscorea alata genotypes were evaluated for their reaction to these diseases in four distinct agro-ecological zones in Nigeria for two years. Data obtained were subjected to li...
Egesi, Chiedozie N.
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Onyeka, T.J.
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Asiedu, Robert
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[Environmental stability of resistance to anthracnose and virus diseases of water yam (Dioscorea alata)]
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Environmental stability of resistance to anthracnose and virus diseases of water yam (Dioscorea alata)