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Blood samples were collected from parasitaemic cattle in the Ghibe Valley, Ethiopia, frozen in liquid nitrogen and transported to Nairobi, Kenya. Twelve of the stabilates were inoculated into individual Boran (Bos indicus) calves and characterised for their sensitivity, in turn, to diminazene ace...


Codjia, V.Mulatu, W.Majiwa, P.A.O.Leak, S.G.A.Rowlands, G.J.Authié, E.D'Ieteren, G.D.M.Peregrine, A.S.[Epidemiology of bovine trypanosomiasis in the Ghibe valley, southwest Ethiopia. 3. Occurrence of population of Trypanosoma congolense resistant to diminazene, isometamidium and homidium]Epidemiology of bovine trypanosomiasis in the Ghibe valley, southwest Ethiopia. 3. Occurrence of population of Trypanosoma congolense resistant to diminazene, isometamidium and homidium

Trypanosomes are covered with a dense layer of antigenically identical glycoproteins. These molecules are highly immunogenic and elicit an antibody response in the infected host. Antibodies against the surface-exposed epitopes of the trypanosome variable surface glycoproteins (VSG) mediate the de...


Williams, D.J.L.Taylor, K.Wilkie, B.Gichuki, B.Authié, E.[Antibody responses to the surface-exposed epitopes of the trypanosome variable surface glycoprotein in N'Dama and Boran cattle]Antibody responses to the surface-exposed epitopes of the trypanosome variable surface glycoprotein in N'Dama and Boran cattle
Authié, E.Muteti, D.K.Williams, D.J.L.[Antibody responses to invariant antigens of Trypanosoma congolense in cattle of differing susceptibility to trypanosomiasis]Antibody responses to invariant antigens of Trypanosoma congolense in cattle of differing susceptibility to trypanosomiasis

Trypanosoma brucei contain a serine oligopeptidase (OP-Tb) that is released into (and remains active in) the blood of trypanosome-infected animals. Here a similar enzyme from Trypanosoma congolense is described. This oligopeptidase, called OP-Tc, was purified using three-phase partitioning, and i...


Morty, R.E.Authié, E.Troeberg, L.Lonsdale-Eccles, J.D.Coetzer, T.H.T.[Purification and characterisation of a typsin-like serine oligo peptidase from Trypanosoma congolense]Purification and characterisation of a typsin-like serine oligo peptidase from Trypanosoma congolense

A 69-kDa immunodominant protein of Trypanosoma congolense was identified as a member of the hsp70 family that is homologous to mammalian BiP. We report here the expression of the gene encoding the T. congolense BiP in a bacterial system. Dot blotting of the truncated recombinant proteins confirme...


Boulange, A.F.Katende, J.Authié, E.[Trypanosoma congolense: Expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis]Trypanosoma congolense: Expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis
Boulange, A.F.Authié, E.[A 69-KDa immunodominant antigen of Trypanosoma (Nannomonas) congolense is homologous to immunoglobulin heavy chain binding protein (Bip)]A 69-KDa immunodominant antigen of Trypanosoma (Nannomonas) congolense is homologous to immunoglobulin heavy chain binding protein (Bip)

Approximately 390 East African Zebu calves from birth to 3 years of age and their dams were monitored monthly from 1986 to 1992 in nine village herds in an area of high trypanosomiasis risk in southwest Ethiopia where there was resistance to all available trypanocidal drugs. Cattle were individua...


Rowlands, G.J.Mulatu, W.Authié, E.D'Ieteren, G.D.M.Leak, S.G.A.Nagda, S.M.[Effects of trypanosomiasis on growth and mortality of young East African Zebu cattle exposed to drug-resistant trypanosomes]Effects of trypanosomiasis on growth and mortality of young East African Zebu cattle exposed to drug-resistant trypanosomes

In order to test the hypothesis that trypanosome cysteine proteinases (CPs) contribute to pathology of trypanosomosis. cattle were immunised with CPl and/or CP2, the major Cps of Trypanosoma congolense, and subsequently challenged with T. congolense. Immunisation had no effect on the establishmen...


Authié, E.Boulange, A.F.Muteti, D.Lalmanach, G.Gauthier, F.Musoke, A.J.[Immunisation of cattle with cysteine proteinases of Trypanosoma congolense: Targetting the disease rather than the parasite]Immunisation of cattle with cysteine proteinases of Trypanosoma congolense: Targetting the disease rather than the parasite

A 69-kDa antigen of Trypanosoma congolense induces both IgM and IgG in experimentally infected cattle. Cloning and sequencing of the gene coding for this antigen revealed that it belongs to the heat shock protein 70 family (hsp70) and is homologous to mammalian immunoglobulin binding protein (BiP...


Boulange, A.F.Katende, J.Authié, E.[A heat shock protein 70 of Trypanosoma congolense as a diagnositic antigen]A heat shock protein 70 of Trypanosoma congolense as a diagnositic antigen

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