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Blood samples were collected from parasitaemic cattle in the Ghibe Valley, Ethiopia, frozen in liquid nitrogen and transported to Nairobi, Kenya. Twelve of the stabilates were inoculated into individual Boran (Bos indicus) calves and characterised for their sensitivity, in turn, to diminazene ace...


Codjia, V.Mulatu, W.Majiwa, P.A.O.Leak, S.G.A.Rowlands, G.J.Authié, E.D'Ieteren, G.D.M.Peregrine, A.S.[Epidemiology of bovine trypanosomiasis in the Ghibe valley, southwest Ethiopia. 3. Occurrence of population of Trypanosoma congolense resistant to diminazene, isometamidium and homidium]Epidemiology of bovine trypanosomiasis in the Ghibe valley, southwest Ethiopia. 3. Occurrence of population of Trypanosoma congolense resistant to diminazene, isometamidium and homidium
Authié, E.Muteti, D.K.Williams, D.J.L.[Antibody responses to invariant antigens of Trypanosoma congolense in cattle of differing susceptibility to trypanosomiasis]Antibody responses to invariant antigens of Trypanosoma congolense in cattle of differing susceptibility to trypanosomiasis

Trypanosoma brucei contain a serine oligopeptidase (OP-Tb) that is released into (and remains active in) the blood of trypanosome-infected animals. Here a similar enzyme from Trypanosoma congolense is described. This oligopeptidase, called OP-Tc, was purified using three-phase partitioning, and i...


Morty, R.E.Authié, E.Troeberg, L.Lonsdale-Eccles, J.D.Coetzer, T.H.T.[Purification and characterisation of a typsin-like serine oligo peptidase from Trypanosoma congolense]Purification and characterisation of a typsin-like serine oligo peptidase from Trypanosoma congolense

A 69-kDa immunodominant protein of Trypanosoma congolense was identified as a member of the hsp70 family that is homologous to mammalian BiP. We report here the expression of the gene encoding the T. congolense BiP in a bacterial system. Dot blotting of the truncated recombinant proteins confirme...


Boulange, A.F.Katende, J.Authié, E.[Trypanosoma congolense: Expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis]Trypanosoma congolense: Expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis
Boulange, A.F.Authié, E.[A 69-KDa immunodominant antigen of Trypanosoma (Nannomonas) congolense is homologous to immunoglobulin heavy chain binding protein (Bip)]A 69-KDa immunodominant antigen of Trypanosoma (Nannomonas) congolense is homologous to immunoglobulin heavy chain binding protein (Bip)

In order to test the hypothesis that trypanosome cysteine proteinases (CPs) contribute to pathology of trypanosomosis. cattle were immunised with CPl and/or CP2, the major Cps of Trypanosoma congolense, and subsequently challenged with T. congolense. Immunisation had no effect on the establishmen...


Authié, E.Boulange, A.F.Muteti, D.Lalmanach, G.Gauthier, F.Musoke, A.J.[Immunisation of cattle with cysteine proteinases of Trypanosoma congolense: Targetting the disease rather than the parasite]Immunisation of cattle with cysteine proteinases of Trypanosoma congolense: Targetting the disease rather than the parasite

A 69-kDa antigen of Trypanosoma congolense induces both IgM and IgG in experimentally infected cattle. Cloning and sequencing of the gene coding for this antigen revealed that it belongs to the heat shock protein 70 family (hsp70) and is homologous to mammalian immunoglobulin binding protein (BiP...


Boulange, A.F.Katende, J.Authié, E.[A heat shock protein 70 of Trypanosoma congolense as a diagnositic antigen]A heat shock protein 70 of Trypanosoma congolense as a diagnositic antigen

The catalytic domains of two closely related cysteine proteinases (CP l and CP2) from Trypanosoma congolense, referred to as Cl and C2, were expressed as proforms in Escherichia coli (C 1) and in the baculovirus system (C I and C2). While the bacterial expression system did not allow recovery of ...


Boulange, A.F.Serveau, C.Brillard, M.Minet, C.Gauthier, F.Diallo, A.Lalmanach, G.Authié, E.[Functional expression of the catalytic domains of two cysteine proteinases from Trypanosoma congolense]Functional expression of the catalytic domains of two cysteine proteinases from Trypanosoma congolense

Congopain and cruzipain, the major cysteine proteinases from Trypanosoma congolense and Trypanosoma cruzi, were compared for their activities towards a series of new, sensitive fluorogenic substrates of the papain family of cysteine proteinases and for their sensitivity to inhibition by cystatins...


Chagas, J.R.Authié, E.Servean, C.Lalmanach, G.Juliano, L.Gauthier, F.[A comparison of the enzymatic properties of the major cysteine proteinases from Trypanosoma congolense and Trypanosoma cruzi]A comparison of the enzymatic properties of the major cysteine proteinases from Trypanosoma congolense and Trypanosoma cruzi

Memory T- and B-cell responses to trypanosome antigens were measured in peripheral blood mononuclear cells, spleen and lymph node cells obtained from 4 trypanotolerant N'Dama cattle which had been exposed to 6 experimental infections with Trypanosoma congolense. These cattle were treated with try...


Lutje, V.Taylor, K.A.Boulange, A.F.Authié, E.[Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle]Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle

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