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Naessens, JanNewson, J.Bensaid, A.Teale, A.J.Magondu, J.Black, S.J.[De novo expression of T-cell markers on Theileria parva-transformed lymphoblasts in cattle]De novo expression of T-cell markers on Theileria parva-transformed lymphoblasts in cattle

Recombinant bovine IFN gamma is a potent inhibitor of Cowdria ruminantium growth in vitro irrespective of the rickettsial stock, or the origin of the endothelial cells. These results suggest an important role for IFN gamma in protective immune responses against C. ruminantium infections. Here we ...


Totte, P.Vachiery, N.Martínez, D.Trap, I.Ballingall, K.T.MacHugh, Niall D.Bensaid, A.Werenne, J.[Recombinant bovine interferon gamma inhibits the growth of Cowdria ruminantium but fails to induce major histocompatibility complex class II following infection of endothelial cells]Recombinant bovine interferon gamma inhibits the growth of Cowdria ruminantium but fails to induce major histocompatibility complex class II following infection of endothelial cells

Understanding the basis of protective immunity to Cowdria ruminantium will facilitate the development of an effective subunit vaccine against heartwater in ruminants and contribute to a better definition of protective immune mechanisms to obligate intracellular pathogens in general. Until recentl...


Totte, P.Bensaid, A.Mahan, S.M.Martínez, D.McKeever, Declan J.[Immune responses to Cowdria ruminantium infections]Immune responses to Cowdria ruminantium infections
Clevers, H.C.MacHugh, Niall D.Bensaid, A.Dunlap, S.Baldwin, C.L.Kaushal, A.Iams, K.P.Howard, C.J.Morrison, W. Ivan[Identification of a bovine surface antigen uniquely expressed on CD4/CD8 negative TCR-gamma/delta positive T lymphocytes]Identification of a bovine surface antigen uniquely expressed on CD4/CD8 negative TCR-gamma/delta positive T lymphocytes

Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromat...


Totte, P.Nyanjui, J.Bensaid, A.McKeever, Declan J.[Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium]Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium

Cattle were successfully immunized against heartwater with a lysate of Cowdria ruminantium formulated in Freund's adjuvant. Vaccinated animals proved fully resistant to virulent challenge 3 and 10 months after vaccination. For the first time a helper T lymphocyte response to Cowdria antigens was ...


Totte, P.McKeever, Declan J.Martínez, D.Bensaid, A.[Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium]Analysis of T-cell responses in cattle immunized against heartwater by vaccination with killed elementary bodies of Cowdria ruminantium
Totte, P.Nyanjui, J.Bensaid, A.McKeever, Declan J.[The use of CD4+ T-cell lines to screen for immunogenic proteins of Cowdria ruminantium]The use of CD4+ T-cell lines to screen for immunogenic proteins of Cowdria ruminantium

The response of peripheral blood mononuclear cells (PBMC) collected from cattle immunized with killed C. ruminantium to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells prolifer...


Totte, P.McKeever, Declan J.Jongejan, F.Barbet, A.Mahan, S.M.Mwangi, D.Bensaid, A.[Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium]Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium

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