The objective of the present study was to compare the ovarian follicular response, cumulus-oocyte complex (COC) collection rate, and maturational status of COC collected from alpacas subsequent to treatment with two different superstimulatory protocols. Alpacas (n = 7 per group) were treated with...
Ratto Fuster, Marcelo
,
Gómez, C.
,
Berland Olea, Marco
,
Adams, G.P.
,
[Effect of ovarian superstimulation on COC collection and maturation in alpacas]
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Effect of ovarian superstimulation on COC collection and maturation in alpacas
Many fish species with economic importance have been heavily exploited, causing the collapse of their fisheries. For this reason, there have been attempts to cultivate fish species for commercial purposes, resulting in varying success. It has been observed that when some species are kept in capti...
Muchas especies de peces de importancia económica han sido intensamente explotadas, provocando el colapso de sus pesquerías. Por este motivo, en algunas de estas especies se han hecho intentos por lograr cultivar individuos con fines comerciales, obteniendo relativo éxito. Se ha observado que cua...
Valdebenito Isler, Iván
,
Paiva, L.
,
Berland Olea, Marco
,
[Atresia folicular en peces teleósteos: Una revisión]
,
[Follicular atresia in teleost fish: A review]
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Follicular atresia in teleost fish: A review
Se comparó el desarrollo in vitro de ovocitos obtenidos de ovarios de vaca de matadero, madurados, fecundados y cultivados in vitro bajo dos sistemas. Los ovocitos fueron cultivados en un medio de maduración a 39 °C, 5 % de CO2 y humedad relativa de 95 % durante 22 horas. Posteriormente, fuer...
The in vitro development of matured and fertilized bovine oocytes was compared between two culture systems. Oocytes were collected by aspiration of follicles of 3-8 mm in diameter using an 18g needle. After morphological selection the oocytes were incubated at 39 0C, 5 % C02 y 95 % relative humid...
Ratto Fuster, Marcelo
,
Berland Olea, Marco
,
Wolter, M.
,
Matamoros, Roberto
,
[Desarrollo de embriones de bovino obtenidos por fecundación in vitro cultivados con células oviductales o medio condicionado y transferidos a hembras receptoras]
,
[Bovine embryo development produced by in vitro fertilization cultured with oviductal cell or conditioned medium and transfer to recipients]
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Bovine embryo development produced by in vitro fertilization cultured with oviductal cell or conditioned medium and transfer to recipients
The aim of this research was to evaluate the effect of Follicle Stimulating Hormone (FSH) treatment on quality and in vitro maturation potential of cumulus-oocyte complexes (COCs) recovered from domestic cats. Twenty-one mature cats were randomly assigned to two groups, FSH (n=9) and Control (n=1...
Sanchez Riquelme, Alfonso
,
Lopez Zamorano, Leonardo
,
Silva Jimenez, Mauricio
,
Berland Olea, Marco
,
[In vitro maturation of cat oocytes obtained from females treated with Follicle Stimulating Hormone]
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In vitro maturation of cat oocytes obtained from females treated with Follicle Stimulating Hormone
The objective was to evaluate the developmental competence of cumulus-oocyte complexes (COC) collected by follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly to two groups (n = 16 per group) and treated, at the time of ovarian follicular wave emergence, with eit...
Berland Olea, Marco
,
Baer, A.
,
Ruíz, J.
,
Parraguez, Víctor
,
Morales, P.
,
Adams, G.P.
,
Ratto Fuster, Marcelo
,
[In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas]
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In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas
Buecher, D. D.
,
Castro, M. A.
,
Silva Jiménez, Mauricio
,
Berland Olea, Marco
,
Concha, I. I.
,
Ratto, Marcelo
,
[GRANULOCYTE-MACROPHAGE COLONY STIMULATNG FACTOR (GM-CSF) ENHANCES CUMULUS CELLS EXPANSION OF IN VITRO-MATURED BOVINE CUMULUS-OOCYTE COMPLEXES IN A CHEMICALLY DEFINED MEDIUM]
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GRANULOCYTE-MACROPHAGE COLONY STIMULATNG FACTOR (GM-CSF) ENHANCES CUMULUS CELLS EXPANSION OF IN VITRO-MATURED BOVINE CUMULUS-OOCYTE COMPLEXES IN A CHEMICALLY DEFINED MEDIUM
Zapata, J.
,
Fernandez, F.
,
Berland Olea, Marco
,
Colazo, M.
,
Peralta, O.
,
Felmer, R.
,
Ratto, Marcelo
,
[EFFECT OF SYNTHETIC OVIDUCT FLUID MEDIUM (SOF) v. OPTIMIZED SIMPLE POTASSIUM MEDIUM (KSOM) ON EMBRYO DEVELOPMENT OF IN VITRO-FERTILIZED BOVINE OOCYTES]
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EFFECT OF SYNTHETIC OVIDUCT FLUID MEDIUM (SOF) v. OPTIMIZED SIMPLE POTASSIUM MEDIUM (KSOM) ON EMBRYO DEVELOPMENT OF IN VITRO-FERTILIZED BOVINE OOCYTES
The objective of the study was to compare the pituitary and ovarian responses after intramuscular, intravenous, or intrauterine administration of P-nerve growth factor (beta-NGF) of seminal plasma origin (SP-NGF) in llamas. In experiment 1, mature female llamas with a growing follicle of 7 mm or ...
Silva Jiménez, Mauricio
,
Fernandez, A.
,
Ulloa Leal, C.
,
Adams, G. P.
,
Berland Olea, Marco
,
Ratto, Marcelo
,
[LH release and ovulatory response after intramuscular, intravenous, and intrauterine administration of beta-nerve growth factor of seminal plasma origin in female llamas]
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LH release and ovulatory response after intramuscular, intravenous, and intrauterine administration of beta-nerve growth factor of seminal plasma origin in female llamas
The type of stimuli triggering GnRH secretion has been used to classify mammalian species into two categories: spontaneous or induced ovulators. In the former, ovarian steroids produced by a mature follicle elicit the release of GnRH from the hypothalamus, but in the latter, GnRH secretion requir...
Ratto, Marcelo H.
,
Berland Olea, Marco
,
Silva Jiménez, Mauricio
,
Adams, Gregg P.
,
[New insights of the role of beta-NGF in the ovulation mechanism of induced ovulating species]
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New insights of the role of beta-NGF in the ovulation mechanism of induced ovulating species