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Plant in vitro vegetative propagation using classical semi-solid culture medium is limited due to the low degree of automation, suboptimal nutrient availability and induced physiological stress which often reduce its efficiency. Temporary Immersion System (TIS) emerged as an innovative approach t...


Jekayinoluwa, T.Gueye, B.Bhattacharjee, R.Osibanjo, O.Shah, T.Abberton, M.T.[Agromorphologic, genetic and methylation profiling of Dioscorea and Musa species multiplied under three micropropagation systems]Agromorphologic, genetic and methylation profiling of Dioscorea and Musa species multiplied under three micropropagation systems

Development of core collection representing the diversity in the entire germplasm creates a better access and enhanced utilization of the main collection thus allowing rapid evaluation in crop improvement programs. Core collections are dynamic in nature and needs revisiting when additional germpl...


Girma Tessema, G.Bhattacharjee, R.López Montes, A.Gueye, B.Ofodile, S.Franco, J.Abberton, M.T.[Re-defining the yam (Dioscorea spp.) core collection using morphological traits]Re-defining the yam (Dioscorea spp.) core collection using morphological traits

Some genetic studies using molecular methods such as diversity assessment or marker-assisted selection require collection of a large number of samples from fields located in the vicinity or in remote areas, followed by isolation of good quality DNA in a short time span. In the present study, diff...


Bhattacharjee, R.Ferguson, M.Gedil, M.Dumet, D.Ingelbrecht, I.L.[Field collection,preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.)]Field collection,preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.)

Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or se...


Sharma, K.Bhattacharjee, R.Sartie, A.M.Kumar, P.L.[An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction]An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction

Screening carotenoids of elite accessions of yam (Dioscorea spp.) used in the global yam breeding program has been conducted to quantitatively determine the carotenoid composition of the crop. Comparisons to previous data reporting cerotenoid levels in yam has been made, in order to deduce greate...


Price, E.J.Bhattacharjee, R.López Montes, A.Fraser, Paul D.[Carotenoid profiling of yams: clarity, comparisons and diversity]Carotenoid profiling of yams: clarity, comparisons and diversity

Introduction Ninety-seven percent of yam (Dioscorea spp.) production takes place in low income food deficit countries (LIFDCs) and the crop provides 200 calories a day to approximately 300 million people. Therefore, yams are vital for food security. Yams have high-yield potential and high market ...


Price, E.J.Bhattacharjee, R.López Montes, A.Fraser, Paul D.[Metabolite profiling of yam (Dioscorea spp.) accessions for use in crop improvement programmes]Metabolite profiling of yam (Dioscorea spp.) accessions for use in crop improvement programmes

Water yam (Dioscorea alata L.) is one of the most important food yams with wide geographical distribution in tropics. One of the major constraints to water yam production is anthracnose disease caused by a fungus, Colletotrichum gloesporioides (Penz.). There are no economically feasible solutions...


Bhattacharjee, R.Nwadili, C.O.Saski, C.A.Agre, A.P.Scheffle, B.E.Augusto, J.López Montes, A.Onyeka, J.T.Kumar, P.L.Bandyopadhyay, Ranajit[An EST-SSR based genetic linkage map and identification of QTLs for anthracnose disease resistance in water yam (Dioscorea alata L.)]An EST-SSR based genetic linkage map and identification of QTLs for anthracnose disease resistance in water yam (Dioscorea alata L.)

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