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Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or se...


Sharma, K.Bhattacharjee, R.Sartie, A.M.Kumar, P.L.[An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction]An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction

Anthracnose, caused by Colletotrichum gloeosporioides, is one of the major constraints limiting water yam (Dioscorea alata) production in the tropics. In this region, yam anthracnose control is mostly achieved by the deployment of moderately resistant yam genotypes. Therefore, screening for new s...


Nwadili, C.O.Augusto, J.Bhattacharjee, R.Atehnkeng, J.López Montes, A.Onyeka, T.J.Kumar, P.L.Asiedu, RobertBandyopadhyay, Ranajit[Comparative reliability of screening parameters for anthracnose resistance in water yam (Dioscorea alata)]Comparative reliability of screening parameters for anthracnose resistance in water yam (Dioscorea alata)

Field and laboratory studies were conducted in Andhra Pradesh, India, to evaluate the effectiveness of a rapid screening method using Perl's Prussian blue stain for screening grain samples from different pearl millet genotypes for zinc (Zn) content. A total of 29 entries representing a wide range...


Velu, G.Bhattacharjee, R.Rai, K.N.Sahrawat, K.L.Longvah, T.[A simple and rapid screening method for grain zinc content in pearl millet]A simple and rapid screening method for grain zinc content in pearl millet

Pigeonpea (Cajanus cajan (L.) Millsp. is one of the most important legume crops as major source for proteins, minerals and vitamins, in addition to its multiple uses as food, feed, fuel, soil enricher, or soil binder, and in fencing, roofing and basket making. ICRISAT's genebank conserves 13,632 ...


Upadhyaya, Hari D.Reddy, K.N.Sharma, S.Varshney, Rajeev K.Bhattacharjee, R.Singh, S.Gowda, C.L.L.[Pigeonpea composite collection and identification of germplasm for use in crop improvement programmes]Pigeonpea composite collection and identification of germplasm for use in crop improvement programmes

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