A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...
Morzaria, S.P.
,
Dolan, T.T.
,
Norval, R.A.I.
,
Bishop, Richard P.
,
Spooner, P.R.
,
[Generation and characterisation of cloned Theileria parva parasites]
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Generation and characterisation of cloned Theileria parva parasites
Allsopp, B.A.
,
Bayles, H.A.
,
Allsopp, M.T.
,
Cavalier-Smith, T.
,
Bishop, Richard P.
,
Carrington, D.M.
,
Sohanpal, B.K.
,
Spooner, P.R.
,
[Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences]
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Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences
Minisatellite tandem repeat elements are well known components of vertebrate genomes, but have not yet been extensively characterized in lower eukaryotes. We describe two unusual. AT-rich minisatellites of the protozoan parasite Theileria parva whose sequences are unrelated to the G/C-rich `chi m...
Bishop, Richard P.
,
Morzaria, S.P.
,
Gobright, E.I.
,
[Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva]
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Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva
Telomere-associated (TA) DNA sequences of the intracellular protozoan parasite Theileria parva were isolated by a novel strategy using a modified version of single-specific-primer polymerase chain reaction (SSP-PCR). Nucleotide sequences of non-coding TA DNA from three telomeres (6017 bp, 2435 bp...
Sohanpal, B.K.
,
Bishop, Richard P.
,
Wasawo, D.
,
[Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats]
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Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats
We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...
Nene, Vishvanath
,
Lee, D.
,
Kang'a, S.
,
Skilton, Robert A.
,
Shah, Tushaar
,
Villiers, Etienne P. de
,
Mwaura, S.
,
Taylor, D.
,
Quackenbush, J.
,
Bishop, Richard P.
,
[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]
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Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index...
Nene, Vishvanath
,
Lee, D.
,
Quackenbush, J.
,
Skilton, Robert A.
,
Mwaura, S.
,
Gardner, M.J.
,
Bishop, Richard P.
,
[AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum]
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AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum
Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...
Bishop, Richard P.
,
Lambson, B.
,
Wells, C.
,
Pandit, P.
,
Osaso, J.
,
Nkonge, C.
,
Morzaria, S.P.
,
Musoke, A.J.
,
Nene, Vishvanath
,
[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]
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A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle
Recent advances in genomic technology have focused many veterinary researchers on the possibility of producing one multivalent recombinant vaccine against all the haemoparasites that infect cattle in the tropics. Before such a vaccine is developed it is essential to define target cattle popula...
Oura, C.A.L.
,
Bishop, Richard P.
,
Wampande, E.M.
,
Lubega, G.W.
,
Tait, A.
,
[Application of a reverse line blot assay to the study of haemoparasites in cattle in Uganda]
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Application of a reverse line blot assay to the study of haemoparasites in cattle in Uganda
Mini- and microsatellite sequences show high levels of variation and therefore provide excellent tools for both the genotyping and population genetic analysis of parasites. Herein we describe the identification of a panel of 11 polymorphic microsatellites and 49 polymorphic minisatellites of th...
Oura, C.A.L.
,
Odongo, David O.
,
Lubega, G.W.
,
Spooner, P.R.
,
Tait, A.
,
Bishop, Richard P.
,
[A panel of microsatellite and minisatellite markers for the characterisation of field isolates of Theileria parva]
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A panel of microsatellite and minisatellite markers for the characterisation of field isolates of Theileria parva
Bishop, Richard P.
,
Sohanpal, B.K.
,
Kariuki, D.
,
Young, A.S.
,
Nene, Vishvanath
,
Baylis, H.
,
Allsopp, B.A.
,
Spooner, P.R.
,
Dolan, T.T.
,
Morzaria, S.P.
,
[Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction]
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Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction