Bacteriophage clones containing Theileria parva genomic DNA derived from two different telomeres were isolated and the nucleotide sequences of the telomeric repeats and adjacent telomere-associated (TAS) DNA were determined. The T. parva telomeric repeat sequences, a tandem array of TTTTAGGG or T...
Sohanpal, B.K.
,
Morzaria, S.P.
,
Gobright, E.I.
,
Bishop, Richard P.
,
[Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome]
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Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome
Allsopp, B.A.
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Bayles, H.A.
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Allsopp, M.T.
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Cavalier-Smith, T.
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Bishop, Richard P.
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Carrington, D.M.
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Sohanpal, B.K.
,
Spooner, P.R.
,
[Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences]
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Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences
Telomere-associated (TA) DNA sequences of the intracellular protozoan parasite Theileria parva were isolated by a novel strategy using a modified version of single-specific-primer polymerase chain reaction (SSP-PCR). Nucleotide sequences of non-coding TA DNA from three telomeres (6017 bp, 2435 bp...
Sohanpal, B.K.
,
Bishop, Richard P.
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Wasawo, D.
,
[Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats]
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Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats
Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index...
Nene, Vishvanath
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Lee, D.
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Quackenbush, J.
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Skilton, Robert A.
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Mwaura, S.
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Gardner, M.J.
,
Bishop, Richard P.
,
[AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum]
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AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum
Several tannin-tolerant bacteria were isolated from enrichment cultures of rumen microflora of bush duiker, giraffe, Grant's gazelle, sheep, and goat, and established in medium containing crude tannin extracts or tannic acid. The isolates were characterized by classical and molecular methods. The...
Odenyo, A.A.
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Bishop, Richard P.
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Asefa, G.
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Jamnadass, Ramni H.
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Odongo, D.
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Osuji, P.O.
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[Characterization of tannin-tolerant bacterial isolates from East African ruminants]
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Characterization of tannin-tolerant bacterial isolates from East African ruminants
Two strains of obligately anaerobic cellulolytic bacteria designated as PS7 and PS8 (PS for porcupine species) were isolated from hindgut fluid of a crested porcupine (Hystrix cristata). The rates of cellulose degradation, total volatile fatty acids, and gas production from cellulose by the isola...
Odenyo, A.A.
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Bishop, Richard P.
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Asefa, G.
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Wells, C.
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Osuji, P.O.
,
[Isolation and characterization of anaerobic cellulose-degrading bacteria from East African porcupine (Hysterix cristata)]
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Isolation and characterization of anaerobic cellulose-degrading bacteria from East African porcupine (Hysterix cristata)
Allsopp, B.A.
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Baylis, H.A.
,
Bishop, Richard P.
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Allsopp, M.T.
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Carrington, D.M.
,
Collins, N.E.
,
[Six different species of Theileria can be diagnosed using specific DNA probes]
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Six different species of Theileria can be diagnosed using specific DNA probes
Polymorphic, multicopy gene families are frequently located in subtelomeric regions of the genomes of parasitic protozoa. Theileria parva telomere-associated (TA) DNA from two chromosomes contained long open reading frames (ORFs) 54% identical at the N-termini whose ends were 2670 and 2680 bp fro...
Bishop, Richard P.
,
Gobright, E.I.
,
Nene, Vishvanath
,
Morzaria, S.P.
,
Musoke, A.J.
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Sohanpal, B.K.
,
[Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres]
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Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres
As a first step in developing compartment-specific markers for protein trafficking within Theileria parva, we have isolated cDNAs encoding homologues of the small GTP binding proteins Rab1 and Rab4. The T. parva homologue of Rab1 (TpRab1), a protein which regulates vesicular transport between the...
Janoo, R.T.K.
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Musoke, A.J.
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Wells, C.
,
Bishop, Richard P.
,
[A Rab1 homologue with a novel isoprenylation signal provides insight into the secretory pathway of Theileria parva]
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A Rab1 homologue with a novel isoprenylation signal provides insight into the secretory pathway of Theileria parva
When compared with other Apicomplexan organisms. Theileria parva has an execptionally small, 10-12 Mbp. genome. There are only 4 chromosomes, each in the Mbp range, and a complete physical map, based on SfiI linking data, is available for each one. A number of genes and cDNAs have been mapped to ...
Nene, Vishvanath
,
Morzaria, S.P.
,
Bishop, Richard P.
,
[Organisation and informational content of the Theileria parva genome]
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Organisation and informational content of the Theileria parva genome