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The genetic diversity of African pigs, whether domestic or wild has not been widely studied and there is very limited published information available. Available data suggests that African domestic pigs originate from different domestication centers as opposed to international commercial breeds. W...


Mujibi, F.D.N.Okoth, Edward A.Cheruiyot, E.K.Onzere, Cynthia K.Bishop, Richard P.Fèvre, Eric M.Thomas, L.Masembe, C.Plastow, G.Rothschild, Max F.[Genetic diversity, breed composition and admixture of Kenyan domestic pigs]Genetic diversity, breed composition and admixture of Kenyan domestic pigs
Morzaria, S.P.Young, J.R.Spooner, P.R.Dolan, T.T.Bishop, Richard P.[Theileria parva: a restriction map and genetic recombination]Theileria parva: a restriction map and genetic recombination

Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73%...


Bishop, Richard P.Shah, TushaarPelle, RogerHoyle, D.Pearson, T.Haines, L.Brass, A.Hulme, H.Graham, S.P.Taracha, E.L.N.Lu, C.Hass, B.Wortman, J.White, O.Gardner, M.J.Nene, VishvanathVilliers, Etienne P. de[Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage]Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLOs). Two isol...


Bishop, Richard P.Spooner, P.R.Sohanpal, B.K.[Genetic fingerprinting of Theileria parva using a telomeric DNA probe]Genetic fingerprinting of Theileria parva using a telomeric DNA probe

Rab genes encode a subgroup of small GTP-binding proteins within the ras super-family that regulate targeting and fusion of transport vesicles within the secretory and endocytic pathways. These genes are of particular interest in the protozoan phylum Apicomplexa, since a family of Rab GTPases has...


Langsley, G.Noort, V. vanCarret, C.Meissner, M.Villiers, Etienne P. deBishop, Richard P.Pain, A.[Comparative genomics of the Rab protein family in Apicomplexan parasites]Comparative genomics of the Rab protein family in Apicomplexan parasites

The intracellular protozoan parasite Theileria parva transforms bovine lymphocytes inducing uncontrolled proliferation. Proteins released from the parasite are assumed to contribute to phenotypic changes of the host cell and parasite persistence. With 85 members, genes encoding subtelomeric varia...


Schmuckli-Maurer, J.Casanova, C.S.Affentranger, S.Kang'a, S.Nene, VishvanathKatzer, F.McKeever, Declan J.Müller, JoachimBishop, Richard P.Arnab Pain, A.Dobbelaere, D.A.E.[Expression analysis of the Theileria parva Subtelomere-encoded Variable Secreted Protein gene family]Expression analysis of the Theileria parva Subtelomere-encoded Variable Secreted Protein gene family

Ticks secrete a cement cone composed of many salivary proteins, some of which are rich in the amino acid glycine in order to attach to their hosts' skin. Glycine-rich proteins (GRPs) are a large family of heterogeneous proteins that have different functions and features; noteworthy are their adhe...


Maruyama, S.R.Anatriello, E.Anderson, J.M.Ribeiro, J.M.Bradao, L.G.Valenzuela, J.G.Ferreira, B.R.García, G.R.Szabo, M.J.P.Patel, S.Bishop, Richard P.Miranda Santos, I.K.F. de[The expression of genes coding for distinct types of glycine-rich proteins varies according to the biology of three metastriate ticks, Rhipicephalus (Boophilus) microplus, Rhipicephalus sanguineus and Amblyomma cajennense]The expression of genes coding for distinct types of glycine-rich proteins varies according to the biology of three metastriate ticks, Rhipicephalus (Boophilus) microplus, Rhipicephalus sanguineus and Amblyomma cajennense

Viral enteritis is a serious problem accounting for deaths in neonatal animals and humans worldwide. The absence of surveillance programs and diagnostic laboratory facilities have resulted in a lack of data on rotavirus associated diarrheas in pigs in East Africa. Here we describe the incidence o...


Amimo, Joshua O.Jung'a, J.O.Ogara, W.O.Vlasova, A.N.Njahira, Moses N.Maina, S.Okoth, Edward A.Bishop, Richard P.Saif, L.J.Djikeng, Appolinaire[Detection and genetic characterization of porcine group A rotaviruses in asymptomatic pigs in smallholder farms in East Africa: Predominance of P[8] genotype resembling human strains]Detection and genetic characterization of porcine group A rotaviruses in asymptomatic pigs in smallholder farms in East Africa: Predominance of P[8] genotype resembling human strains

The role of the ancestral sylvatic cycle of the African swine fever virus (ASFV) is not well understood in the endemic areas of eastern Africa. We therefore analysed the ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Ornithodorus porcinus ...


Gallardo, C.Okoth, S.Pelayo, V.Anchuelo, R.Martin, E.Simon, A.Llorente, A.Nieto, R.Soler Garzón, AlvaroMartin, R.Arias, MarisaBishop, Richard P.[African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site]African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site

Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2,000 to 5,000 EAT2 genome equivalents...


Bishop, Richard P.Obura, M.Odongo, D.Odenyo, A.A.[Specific PCR assay for a tannin-tolerant Selenomonas ruminantium isolate, derived from helicase coding sequences]Specific PCR assay for a tannin-tolerant Selenomonas ruminantium isolate, derived from helicase coding sequences

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