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Theileria parva, a tick-borne api-complexan protozoan parasite, causes East Coast fever (ECF) in cattle. Control of the disease by improved vaccination is believed to provide a sustainable solution. Class I MHC-restricted CD8+ cytotoxic T lymphocytes (CTL) directed at schizontinfected cells const...
In this paper genomic research is discussed with regard to prospects for improving livestock productivity. Topics of discussion include, constraints to livestock productivity in developing countries; biotechnology research at ILRI; improvement of rumen fermentation; livestock genetic resources an...
Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2,000 to 5,000 EAT2 genome equivalents...
We describe the development of TparvaDB, a comprehensive resource to facilitate research towards development of an East Coast fever vaccine, by providing an integrated user-friendly database of all genome and related data currently available for Theileria parva. TparvaDB is based on the Generic M...
The "Muguga cocktail" live vaccine comprises three Theileria parva stocks (Muguga, Kiambu 5 and the buffalo-derived Serengeti-transformed) and has been used extensively in Eastern, Central and Southern Africa with an infection and treatment protocol to protect cattle against East Coast fever. We ...
Molecular tools based on seminested RFLP-PCR techniques to characterize field parasites in bloodspots dried on filter paper permitted investigation of the extent and the dynamics of diversity of Theileria parva populaitons in the field. Parallel molecular studies explored the long-term genome sta...
A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous control, has been developed and validated by four National Reference Laboratories of the European Union for Af...
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