Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...
Bishop, Richard P.
,
Nene, Vishvanath
,
Staeyert, J.
,
Rowlands, G.J.
,
Nyanjui, J.
,
Osaso, J.
,
Morzaria, S.P.
,
Musoke, A.J.
,
[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]
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Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
,
Rowlands, G.J.
,
Nene, Vishvanath
,
Nyanjui, J.
,
Katende, J.
,
Spooner, P.R.
,
Mwaura, S.
,
Odongo, D.
,
Nkonge, C.
,
Mbogo, S.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
A cross-sectional survey was carried out in four counties of Jonglei State, South Sudan, between May and June 2012 to determine the distribution and northern limit of Theileria parva, the causative agent of East Coast fever in cattle, and its tick vector Rhipicephalus appendiculatus, as a prerequ...
Marcellino, Wani L.
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Salih, Diaeldin A.
,
Njahira, Moses N.
,
Ndiwa, Nicholas N.
,
Araba, A.
,
El Hussein, A.M.
,
Seitzer, U.
,
Ahmed, J.S.
,
Bishop, Richard P.
,
Skilton, Robert A.
,
[The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation using molecular and serological diagnostic tools]
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The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation using molecular and serological diagnostic tools
The preparation of Theileria parva stabilates has been described by Cunningham et al (1973). The method is detailed in the FAO report (1978) and the FAO tick and tick-borne Disease Manual (1984). Theileria parva stabilates are a source of viable sporozoites that can be used to immunise cattle by ...
Morzaria, S.P.
,
Spooner, P.R.
,
Bishop, Richard P.
,
Mwaura, S.
,
[The preparation of a composite stabilate for immunisation against East Coast fever]
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The preparation of a composite stabilate for immunisation against East Coast fever
Rhipicephalus appendiculatus is an important tick vector of several pathogens and parasitizes domestic and wild animals across eastern and southern Africa. However, its inherent genetic variation and population structure is poorly understood. To investigate whether mammalian host species, geograp...
Kanduma, E.G.
,
Mwacharo, Joram M.
,
Mwaura, S.
,
Njuguna, J.N.
,
Nzuki, I.
,
Kinyanjui, P.W.
,
Githaka, Naftaly W.
,
Heyne, H.
,
Hanotte, Olivier H.
,
Skilton, Robert A.
,
Bishop, Richard P.
,
[Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya]
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Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya
Bovine tuberculosis in domestic livestock such as cattle is an economically important disease with zoonotic potential, particularly in countries with emerging economies. We discuss the findings of recent epidemiological and immunological studies conducted in Ethiopia on host susceptibility differ...
Vordermeier, M.
,
Ameni, G.
,
Berg, S.
,
Bishop, Richard P.
,
Robertson, B.D.
,
Aseffa, A.
,
Hewinson, R.G.
,
Young, D.B.
,
[The influence of cattle breed on susceptibility to bovine tuberculosis in Ethiopia]
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The influence of cattle breed on susceptibility to bovine tuberculosis in Ethiopia
Heterologous priming-boosting vaccination regimens involving priming with plasmid DNA antigen constructs and inoculating (boosting) with the same recombinant antigen expressed in replication-attenuated poxviruses have recently been demonstrated to induce immunity, based on CD4(+)- and CD8(+)-T-c...
Nene, Vishvanath
,
Bishop, Richard P.
,
Quackenbush, J.
,
Pertea, M.
,
Salzberg, S.L.
,
Taracha, E.A.
,
Morzaria, S.P.
,
Fraser, C.M.
,
Gardner, M.
,
[Genomics of Theileria parva]
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Genomics of Theileria parva
Rowlands, G.J.
,
Musoke, A.J.
,
Nene, Vishvanath
,
Bishop, Richard P.
,
Nagda, S.M.
,
Morzaria, S.P.
,
[Application of principal component analysis for classifying East Coast fever reactions in cattle challenged with Theileria parva]
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Application of principal component analysis for classifying East Coast fever reactions in cattle challenged with Theileria parva
Bishop, Richard P.
,
Geysen, D.
,
Skilton, Robert A.
,
Odongo, D.
,
Nene, Vishvanath
,
Allsopp, B.A.
,
Mbogo, S.
,
Spooner, P.R.
,
Morzaria, S.P.
,
[Genomic polymorphism, sexual recombination and molecular epidemiology of Theileria parva]
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Genomic polymorphism, sexual recombination and molecular epidemiology of Theileria parva