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A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the...


Fernández Pinero, J.Gallardo, C.Elizalde, M.Robles, A.Gómez, C.Bishop, Richard P.Heath, L.Couacy-Hyman, E.Fasina, F.O.Pelayo, V.Soler Garzón, AlvaroArias, Marisa[Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library]Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library

Diagnostic methods allowing for rapid identification of pathogens are crucial for controlling and preventing dissemination after disease outbreaks as well as for use in surveillance programs. For arboviruses, detection of the presence of virus in their arthropod hosts is important for monitoring ...


Mwaengo, D.Lorenzo, G.Iglesias, J.Warigia, M.Sang, R.Bishop, Richard P.Brun, A.[Detection and identification of Rift Valley fever virus in mosquito vectors by quantitative real-time PCR]Detection and identification of Rift Valley fever virus in mosquito vectors by quantitative real-time PCR

Zubair, S.Villiers, Etienne P. deYounan, M.Andersson, G.Tettelin, H.Riley, D.R.Jores, JoergBongcam-Rudloff, E.Bishop, Richard P.[Genome sequences of two pathogenic Streptococcus agalactiae isolates from the one-humped camel Camelus dromedarius]Genome sequences of two pathogenic Streptococcus agalactiae isolates from the one-humped camel Camelus dromedarius

Theileria parva is a parasitic protozoan that causes East Coast fever (ECF), an economically important disease of cattle in eastern, central and southern Africa. In South Sudan, ECF is considered a major constraint for livestock development in regions where the disease is endemic. To obtain insig...


Salih, Diaeldin A.Mwacharo, Joram M.Pelle, RogerNjahira, Moses N.Odongo, David O.Mbole-Kariuki, Mary N.Marcellino, Wani L.Malak, Agol K.Kiara, Henry K.El Hussein, A.R.M.Bishop, Richard P.Skilton, Robert A.[Genetic diversity and population structure of Theileria parva in South Sudan]Genetic diversity and population structure of Theileria parva in South Sudan

The central variable region (CVR) within the B602L gene of the African swine fever virus (ASFV) is highly polymorphic within the 23 ASFV genotypes defined by sequencing of the C-terminal end of the p72 locus. Sequencing the p54 gene further discriminates ASFV genotypes that are conserved at the p...


Onzere, Cynthia K.Bastos, A.D.S.Okoth, Edward A.Lichoti, J.K.Nyambego, Edwina B.Owido, M.G.Ndambuki, Gideon M.Bronsvoort, B.M. de C.Bishop, Richard P.[Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011–2013) reveals rapid B602L central variable region evolution]Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011–2013) reveals rapid B602L central variable region evolution

Samples from infected domestic pigs associated with an outbreak of African swine fever (ASF) in three districts of central Uganda in 2007 were confirmed as being infected with African swine fever virus (ASFV) using a P72 gene-based polymerase chain reaction amplification (PCR) assay combined with...


Gallardo, C.Ademun, A.R.Nieto, R.Nantima, NoelinaArias, MarisaMartin, E.Pelayo, V.Bishop, Richard P.[Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007]Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007

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