We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from tic...
Nene, Vishvanath
,
Lee, D.
,
Kang'a, S.
,
Skilton, Robert A.
,
Shah, Tushaar
,
Villiers, Etienne P. de
,
Mwaura, S.
,
Taylor, D.
,
Quackenbush, J.
,
Bishop, Richard P.
,
[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]
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Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index...
Nene, Vishvanath
,
Lee, D.
,
Quackenbush, J.
,
Skilton, Robert A.
,
Mwaura, S.
,
Gardner, M.J.
,
Bishop, Richard P.
,
[AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum]
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AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum
The transcriptional control of gene expression is not well documented in the Arthropoda. We describe transcriptional analysis of two exceptionally divergent homologues (Ra86) of the Bm86 gut antigen from Rhipicephalus appendiculatus. Bm86 forms the basis of a commercial vaccine for the control of...
Kamau, L.
,
Skilton, Robert A.
,
Odongo, David O.
,
Mwaura, S.
,
Githaka, Naftaly W.
,
Kanduma, E.G.
,
Obura, M.
,
Kabiru, E.
,
Orago, A.
,
Musoke, A.J.
,
Bishop, Richard P.
,
[Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)]
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Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)
Biological differences, including vector competence for the protozoan parasite Theileria parva have been reported among populations of Rhipicephalus appendiculatus (Acari: Ixodidae) from different geographic regions. However, the genetic diversity and population structure of this important tick v...
Kanduma, E.G.
,
Mwacharo, Joram M.
,
Sunter, J.D.
,
Nzuk, I.
,
Mwaura, S.
,
Kinyanjui, P.W.
,
Kibe, M.
,
Heyne, H.
,
Hanotte, Olivier H.
,
Skilton, Robert A.
,
Bishop, Richard P.
,
[Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus]
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Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus
Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs ...
Lambson, B.
,
Nene, Vishvanath
,
Obura, M.
,
Shah, Tushaar
,
Pandit, P.
,
Ole-MoiYoi, O.K.
,
Delroux, K.
,
Welburn, S.C.
,
Skilton, Robert A.
,
Villiers, Etienne P. de
,
Bishop, Richard P.
,
[Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells]
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Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells