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Morzaria, S.P.Young, J.R.Spooner, P.R.Dolan, T.T.Bishop, Richard P.[Theileria parva: a restriction map and genetic recombination]Theileria parva: a restriction map and genetic recombination

The infection-and-treatment method (ITM) for immunization of cattle against East Coast fever has historically been used only on a limited scale because of logistical and policy constraints. Recent large-scale deployment among pastoralists in Tanzania has stimulated demand. Concurrently, a suite o...


Giulio, Giuseppe diLynen, GodelieveMorzaria, S.P.Oura, C.Bishop, Richard P.[Live immunization against East Coast fever - current status]Live immunization against East Coast fever - current status

Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73%...


Bishop, Richard P.Shah, TushaarPelle, RogerHoyle, D.Pearson, T.Haines, L.Brass, A.Hulme, H.Graham, S.P.Taracha, E.L.N.Lu, C.Hass, B.Wortman, J.White, O.Gardner, M.J.Nene, VishvanathVilliers, Etienne P. de[Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage]Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...


Toye, Philip G.Gobright, E.I.Nyanjui, J.Nene, VishvanathBishop, Richard P.[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva

Bacteriophage clones containing Theileria parva genomic DNA derived from two different telomeres were isolated and the nucleotide sequences of the telomeric repeats and adjacent telomere-associated (TAS) DNA were determined. The T. parva telomeric repeat sequences, a tandem array of TTTTAGGG or T...


Sohanpal, B.K.Morzaria, S.P.Gobright, E.I.Bishop, Richard P.[Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome]Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome

A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...


Morzaria, S.P.Dolan, T.T.Norval, R.A.I.Bishop, Richard P.Spooner, P.R.[Generation and characterisation of cloned Theileria parva parasites]Generation and characterisation of cloned Theileria parva parasites

Minisatellite tandem repeat elements are well known components of vertebrate genomes, but have not yet been extensively characterized in lower eukaryotes. We describe two unusual. AT-rich minisatellites of the protozoan parasite Theileria parva whose sequences are unrelated to the G/C-rich `chi m...


Bishop, Richard P.Morzaria, S.P.Gobright, E.I.[Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva]Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva

Telomere-associated (TA) DNA sequences of the intracellular protozoan parasite Theileria parva were isolated by a novel strategy using a modified version of single-specific-primer polymerase chain reaction (SSP-PCR). Nucleotide sequences of non-coding TA DNA from three telomeres (6017 bp, 2435 bp...


Sohanpal, B.K.Bishop, Richard P.Wasawo, D.[Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats]Cloning of telomere-associated DNA using single-specific-primer polymerase chain reaction provides evidence for a conserved sequence directly adjacent to Theileria parva telomeric repeats

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

Recent advances in genomic technology have focused many veterinary researchers on the possibility of producing one multivalent recombinant vaccine against all the haemoparasites that infect cattle in the tropics. Before such a vaccine is developed it is essential to define target cattle popula...


Oura, C.A.L.Bishop, Richard P.Wampande, E.M.Lubega, G.W.Tait, A.[Application of a reverse line blot assay to the study of haemoparasites in cattle in Uganda]Application of a reverse line blot assay to the study of haemoparasites in cattle in Uganda

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