?1-pyrroline-5-carboxylate synthetase (P5CS) is the rate-limiting enzyme involved in the biosynthesis of proline in plants. By the 3? rapid amplification of cDNA ends (3?-RACE) approach, a 2,246-bp cDNA sequence was obtained from common bean (Phaseolus vulgaris L.), denominated PvP5CS2 differing ...
Chen, J.
,
Zhang, X.
,
Jing, R
,
Blair, Matthew W.
,
Mao, X
,
Wang, S.
,
[Cloning and genetic diversity analysis of a new P5CS gene from common bean (Phaseolus vulgaris L.)]
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Cloning and genetic diversity analysis of a new P5CS gene from common bean (Phaseolus vulgaris L.)
Single nucleotide polymorphisms (SNPs) are the most common sequence difference found in plant genomes, yet they have not been widely exploited for producing molecular markers in common bean (Phaseolus vulgaris L.). The objective of this study was to develop a SNP assay based on a type of heterodu...
Galeano Mendoza, CH
,
Gómez, M.
,
Rodríguez, L.M.
,
Blair, Matthew W.
,
[CEL I nuclease digestion for SNP discovery and marker development in common bean (Phaseolus vulgaris L.)]
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CEL I nuclease digestion for SNP discovery and marker development in common bean (Phaseolus vulgaris L.)
Asfaw Amele, A.
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Blair, Matthew W.
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Almekinders, Conny J.M.
,
[Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces from the East African highlands]
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Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces from the East African highlands
Background:
Iron is an important micronutrient for all living organisms. Almost 25% of the world population is affected by iron deficiency, a leading cause of anemia. In plants, iron deficiency leads to chlorosis and reduced yield. Both animals and plants may suffer from iron deficiency when thei...
Cvitanich, C
,
Przybylowicz, WJ
,
Urbanski, DF
,
Jurkiewicz, AM
,
Mesjasz-Przybylowicz, J
,
Blair, Matthew W.
,
Astudillo, Carolina
,
Jensen, E
,
Stougaard, J
,
[Iron and ferritin accumulate in separate cellular locations in Phaseolus seeds]
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Iron and ferritin accumulate in separate cellular locations in Phaseolus seeds
Common bean (Phaseolus vulgaris L.) is an important source of dietary protein and minerals worldwide. Genes conditioning variability for mineral contents are not clearly understood. Our ultimate goal is to identify genes conditioning genetic variation for Zn and Fe content. To establish mapping p...
Talukder, ZL
,
Anderson, E
,
Miklas, PN
,
Blair, Matthew W.
,
Osorno Gonzalez, JM
,
Dilawan, M
,
Hossain, KG
,
[Genetic diversity and selection of genotypes to enhance Zn and Fe content in common bean]
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Genetic diversity and selection of genotypes to enhance Zn and Fe content in common bean
Globally, 800 million people are malnourished. Heavily subsidised farmers in rich countries produce sufficient surplus food to feed the hungry, but not at a price the poor can afford. Even donating the rich world’s surplus to the poor would not solve the problem. Most poor people earn their livin...
Broughton, W.J.
,
Hernández, G.
,
Blair, Matthew W.
,
Beebe, Stephen E.
,
Gepts, Paul L.
,
Vanderleyden, J
,
[Beans (Phaseolus spp.)- model food legumes]
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Beans (Phaseolus spp.)- model food legumes
Genetic diversity of populations stored ex situ or in situ can be altered due to the management practices they are subjected to. In this paper, we compare populations of two common bean (Phaseolus vulgaris L.) landraces grown on farms with material collected from the same farms and now kept in tw...
Gómez, O.J.
,
Blair, Matthew W.
,
Frankow-Lindberg, BE
,
Gullberg, U
,
[Comparative study of common bean (Phaseolus vulgaris L.) landraces conserved ex situ in genebanks and in situ by farmers]
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Comparative study of common bean (Phaseolus vulgaris L.) landraces conserved ex situ in genebanks and in situ by farmers
Blair, Matthew W.
,
Astudillo, Carolina
,
Beebe, Stephen E.
,
Roa Velasco, J.I.
,
Kimani, P.M.
,
Chirwa, Rowland
,
[Biofortification breeding of common bean (Phaseolus vulgaris L.)]
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Biofortification breeding of common bean (Phaseolus vulgaris L.)
Background:
Common bean is an important legume crop with only a moderate number of short expressed sequence tags (ESTs) made with traditional methods. The goal of this research was to use full-length cDNA technology to develop ESTs that would overlap with the beginning of open reading frames and ...
Blair, Matthew W.
,
Fernández, A.C.
,
Ishitani, Manabu
,
Moreta Mejia, DE
,
Seki, M
,
Ayling, S.
,
Shinozaki, K
,
[Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)]
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Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)
Breeding for resistance to biotic and abiotic stresses of global importance in common bean is reviewed with emphasis on development and application of marker-assisted selection (MAS). The implementation and adoption of MAS in breeding for disease resistance is advanced compared to the implementat...
Miklas, PN
,
Kelly, James D.
,
Beebe, Stephen E.
,
Blair, Matthew W.
,
[Common bean breeding for resistance against biotic and abiotic stresses : From classical to MAS breeding]
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Common bean breeding for resistance against biotic and abiotic stresses : From classical to MAS breeding