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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

Despite the worldwide importance of cultivated cassava (M. esculenta Crantz) its origin and taxonomic relationships with other species in the genus have not been clearly established. We evaluated a representative sample of the crop’s diversity and six wild taxa with AFLPs to estimate genetic rela...


Roa, ACMaya, M.M.Duque E., Myriam CristinaTohme, Joseph M.Allem, ACBonierbale, Merideth W.[AFLP analysis of relationships among cassava and other Manihot species]AFLP analysis of relationships among cassava and other Manihot species

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties o...


Sánchez, G.M.Restrepo, S.Duque E., Myriam CristinaFregene, Martin A.Bonierbale, Merideth W.Verdier, Valérie[AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)]AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)
Thro, Ann MarieBeachy, Roger N.Bonierbale, Merideth W.Fauquet, Claude M.Henry, GuyHenshaw, Graham G.Hughes, Monica A.Kawano, KazuoRaemakers, C.J.J.M.Roca, William M.Sch"pke, C.Taylor, Nigel J.Visser, Richard G.F.[International research on biotechnology of cassava (tapioca, Manihot esculenta Crantz) and its relevance to Southeast Asian economies: a cassava biotechnology network review]International research on biotechnology of cassava (tapioca, Manihot esculenta Crantz) and its relevance to Southeast Asian economies: a cassava biotechnology network review
Giraldo Zapata, Martha C.Ocampo Nahar, César H.Bravo, EnriqueBonierbale, Merideth W.Debouck, Daniel G.[Polimorfismo isoenzimático corrobora clasificación etnobotánica y distribución de la diversidad de yuca en Colombia]Polimorfismo isoenzimático corrobora clasificación etnobotánica y distribución de la diversidad de yuca en Colombia

Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The n...


Roa, ACChavarriaga Aguirre, PaulDuque E., Myriam CristinaMaya, M.M.Bonierbale, Merideth W.Iglesias, C.A.Tohme, Joseph M.[Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship]Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship

Cassava is an important energy-rich food for millions of people in tropical developing countries. The crop has reached that status after thousands of years of evolution under diverse agro-ecosystems, movement of germplasm within and across continents, and more recently, through scientific genetic...


Iglesias F., Carlos ArielBonierbale, Merideth W.El-Sharkawy, Mabrouk A.Lozano T., José CarlosBellotti, Anthony C.Wheatley, Christopher C.[Focusing basic research for cassava varietal improvement]Focusing basic research for cassava varietal improvement

Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping...


Chavarriaga Aguirre, PaulMaya, M.M.Bonierbale, Merideth W.Kresovich, SFregene, Martin A.Tohme, Joseph M.Kochert, G[Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability]Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability

The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme p...


Chavarriaga Aguirre, PaulMaya, M.M.Tohme, Joseph M.Duque E., Myriam CristinaIglesias, C.A.Bonierbale, Merideth W.Kresovich, SKochert, G[Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections]Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections

Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 × C...


Jorge, VFregene, Martin A.Duque E., Myriam CristinaBonierbale, Merideth W.Tohme, Joseph M.Verdier, Valérie[Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)]Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)

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