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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties o...


Sánchez, G.M.Restrepo, S.Duque E., Myriam CristinaFregene, Martin A.Bonierbale, Merideth W.Verdier, Valérie[AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)]AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)

Simon, R.Carhuapoma, P.Hualla, V.Ghislain, M.Mendiburu, F. deGómez, R.Zorrilla, C.Roca, W.Haan, Stef deBonierbale, Merideth W.[Representing SSR molecular marker profiles using concepts from Andean khipus.]Representing SSR molecular marker profiles using concepts from Andean khipus.

Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The n...


Roa, ACChavarriaga Aguirre, PaulDuque E., Myriam CristinaMaya, M.M.Bonierbale, Merideth W.Iglesias, C.A.Tohme, Joseph M.[Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship]Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship

The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme p...


Chavarriaga Aguirre, PaulMaya, M.M.Tohme, Joseph M.Duque E., Myriam CristinaIglesias, C.A.Bonierbale, Merideth W.Kresovich, SKochert, G[Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections]Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections

Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...


Fregene, Martin A.Okogbenin, EmmanuelMba, CAngel, F.Suarez, MCGutiérrez A., Janeth PatriciaChavarriaga Aguirre, PaulRoca, William M.Bonierbale, Merideth W.Tohme, Joseph M.[Genome mapping in cassava improvement: Challenges, achievements and opportunities]Genome mapping in cassava improvement: Challenges, achievements and opportunities

A preliminary linkage map of cassava (Manihot esculenta Crantz) to increase the efficiency of introgression of specific chromosomes or chromosome fragments from exotic cassava germplasm into various genetic backgrounds and localization of genes of interest in cassava improvement schemes has been ...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Maya, M.M.Bonierbale, Merideth W.Tohme, Joseph M.Iglesias, C.A.[A linkage map of cassava (Manihot esculenta Crantz) based on RFLP and RAPD markers]A linkage map of cassava (Manihot esculenta Crantz) based on RFLP and RAPD markers

The genetic resources available for cassava improvement include advanced breeding lines, collected landraces and the crops`s wild relatives. Information about the structure of these gene pools is important in the development of sound germplasm conservation and use strategies. RAPD markers have be...


Bonierbale, Merideth W.Maya, M.M.Claroz, José LuisIglesias, C.A.[Application of molecular markers to describing the genetic structure of cassava gene pools]Application of molecular markers to describing the genetic structure of cassava gene pools

Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Se...


Gómez, R.Angel, F.Bonierbale, Merideth W.Rodríguez, F.Tohme, Joseph M.Roca, William M.[Selecting heterozygous parents and single-dose markers for genetic mapping in cassava]Selecting heterozygous parents and single-dose markers for genetic mapping in cassava

Haan, Stef deNunez, J.Bonierbale, Merideth W.Ghislain, M.Maesen, J. van der[A Simple Sequence Repeat (SSR) marker comparison of a large in- and ex-situ potato landrace cultivar collection from Peru reaffirms the complementary nature of both conservation strategies.]A Simple Sequence Repeat (SSR) marker comparison of a large in- and ex-situ potato landrace cultivar collection from Peru reaffirms the complementary nature of both conservation strategies.

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