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A 69-kDa immunodominant protein of Trypanosoma congolense was identified as a member of the hsp70 family that is homologous to mammalian BiP. We report here the expression of the gene encoding the T. congolense BiP in a bacterial system. Dot blotting of the truncated recombinant proteins confirme...


Boulange, A.F.Katende, J.Authié, E.[Trypanosoma congolense: Expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis]Trypanosoma congolense: Expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis
Boulange, A.F.Authié, E.[A 69-KDa immunodominant antigen of Trypanosoma (Nannomonas) congolense is homologous to immunoglobulin heavy chain binding protein (Bip)]A 69-KDa immunodominant antigen of Trypanosoma (Nannomonas) congolense is homologous to immunoglobulin heavy chain binding protein (Bip)

In order to test the hypothesis that trypanosome cysteine proteinases (CPs) contribute to pathology of trypanosomosis. cattle were immunised with CPl and/or CP2, the major Cps of Trypanosoma congolense, and subsequently challenged with T. congolense. Immunisation had no effect on the establishmen...


Authié, E.Boulange, A.F.Muteti, D.Lalmanach, G.Gauthier, F.Musoke, A.J.[Immunisation of cattle with cysteine proteinases of Trypanosoma congolense: Targetting the disease rather than the parasite]Immunisation of cattle with cysteine proteinases of Trypanosoma congolense: Targetting the disease rather than the parasite

The catalytic domains of two closely related cysteine proteinases (CP l and CP2) from Trypanosoma congolense, referred to as Cl and C2, were expressed as proforms in Escherichia coli (C 1) and in the baculovirus system (C I and C2). While the bacterial expression system did not allow recovery of ...


Boulange, A.F.Serveau, C.Brillard, M.Minet, C.Gauthier, F.Diallo, A.Lalmanach, G.Authié, E.[Functional expression of the catalytic domains of two cysteine proteinases from Trypanosoma congolense]Functional expression of the catalytic domains of two cysteine proteinases from Trypanosoma congolense

Memory T- and B-cell responses to trypanosome antigens were measured in peripheral blood mononuclear cells, spleen and lymph node cells obtained from 4 trypanotolerant N'Dama cattle which had been exposed to 6 experimental infections with Trypanosoma congolense. These cattle were treated with try...


Lutje, V.Taylor, K.A.Boulange, A.F.Authié, E.[Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle]Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle

Trypanosomiasis is a serious constraint to livestock production in sub-Saharan Africa. Some breeds of cattle are genetically more resistant to the pathogenic effects of trypanosome infection. We measured B-cell activation and the quantity and isotype of antibody produced at the cellular level in ...


Taylor, K.A.Lutje, V.Kennedy, D.Authié, E.Boulange, A.F.Logan-Henfrey, L.L.Gichuki, B.Gettinby, G.[Trypanosoma congolense: B-Lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle]Trypanosoma congolense: B-Lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle

A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmitted Trypanosoma congolense was conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferat...


Lutje, V.Taylor, K.A.Kennedy, D.Authié, E.Boulange, A.F.Gettinby, G.[Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection]Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection

T-cell-mediated immune responses to defined antigens of Trypanosoma congolense were measured in cattle undergoing primary infection. The antigens used were the variable surface glycoprotein and two invariant antigens, a 33-kDa cysteine protease (congopain) and a recombinant form of a 69-kDa heat-...


Lutje, V.Mertens, B.Boulange, A.F.Williams, D.J.L.Authié, E.[Trypanosoma congolense: Proliferative responses and interleukin production in lymph node cells of infected cattle]Trypanosoma congolense: Proliferative responses and interleukin production in lymph node cells of infected cattle

African animal trypanosomosis (nagana) is arguably the most important parasitic disease affecting livestock in sub-Saharan Africa. Since none of the existing control measures are entirely satisfactory, vaccine development is being actively pursued. However, due to antigenic variation, the quest f...


Boulange, A.F.Khamadi, S.A.Pillay, D.Coetzer, T.H.T.Authié, E.[Production of congopain, the major cysteine protease of Trypanosoma (Nannomonas) congolense, in Pichia pastoris reveals unexpected dimerization at physiological Ph]Production of congopain, the major cysteine protease of Trypanosoma (Nannomonas) congolense, in Pichia pastoris reveals unexpected dimerization at physiological Ph

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