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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

Castañeda-Méndez, Oscar F.Ogawa, SatoshiMedina, AdrianaChavarriaga Aguirre, PaulSelvaraj, Michael Gomez[A simple hydroponic hardening system and the effect of nitrogen source on the acclimation of in vitro cassava (Manihot esculenta Crantz)]A simple hydroponic hardening system and the effect of nitrogen source on the acclimation of in vitro cassava (Manihot esculenta Crantz)

Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The n...


Roa, ACChavarriaga Aguirre, PaulDuque E., Myriam CristinaMaya, M.M.Bonierbale, Merideth W.Iglesias, C.A.Tohme, Joseph M.[Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship]Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship

The angiosperm circadian clock has been well established from molecular-genetic studies in a temperate plant model. Conservation of clock function is less explored in plants from the tropics. Cassava (Manihot esculenta) is a staple crop grown in the tropics that has been of limited research inter...


Adeyemo, O. SarahKolmos, ETohme, Joseph M.Chavarriaga Aguirre, PaulFregene, Martin A.Davis, S.J.[Identification and characterization of the Cassava core-clock gene Early Flowering 4]Identification and characterization of the Cassava core-clock gene Early Flowering 4

Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction ...


Murillo-Gómez, Paola AndreaHoyos S, RodrigoChavarriaga Aguirre, Paul[Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] = Organogénesis in-vitro usando tres tipos de tejidos de tomate de árbol [Solanum betaceum (Cav.)]]Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] = Organogénesis in-vitro usando tres tipos de tejidos de tomate de árbol [Solanum betaceum (Cav.)]

The importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. Plant biotechnology offers a wide range of opportunities that can help cassava become a b...


Chavarriaga Aguirre, PaulBrand, AlejandroMedina, AdrianaPrías, MónicaEscobar Pérez, Roosevelt H.Martínez, JuanDíaz, PaulaLópez Carrascal, Camilo ErnestoRoca, William M.Tohme, Joseph M.[The potential of using biotechnology to improve cassava: a review]The potential of using biotechnology to improve cassava: a review

Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping...


Chavarriaga Aguirre, PaulMaya, M.M.Bonierbale, Merideth W.Kresovich, SFregene, Martin A.Tohme, Joseph M.Kochert, G[Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability]Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability

Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accum...


Welsch, RArango, JacoboBar, CSalazar, BAl-Babili, S.Beltrán Giraldo, Jorge AlonsoChavarriaga Aguirre, PaulCeballos, H.Tohme, Joseph M.Beyer, Peter[Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W]]Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W]
Beltrán Giraldo, Jorge AlonsoJaimes, HEcheverry, MLadino Franco, YJLópez, D.Duque E., Myriam CristinaChavarriaga Aguirre, PaulTohme, Joseph M.[Quantitative analysis of transgenes in cassava plants using real-time PCR technology]Quantitative analysis of transgenes in cassava plants using real-time PCR technology

Cassava (Manihot esculenta Crantz) is a vitally important food source for many people in developing tropical countries. There are significant opportunities for improving the compositional qualities and pest resistance of cassava, and modern biotechnology is expected to play an important role in t...


Halsey, MEOlsen, KLTaylor, Nigel J.Chavarriaga Aguirre, Paul[Reproductive biology of cassava (Manihot esculenta Crantz) and isolation of experimental field trials]Reproductive biology of cassava (Manihot esculenta Crantz) and isolation of experimental field trials

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