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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The n...


Roa, ACChavarriaga Aguirre, PaulDuque E., Myriam CristinaMaya, M.M.Bonierbale, Merideth W.Iglesias, C.A.Tohme, Joseph M.[Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship]Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship

The angiosperm circadian clock has been well established from molecular-genetic studies in a temperate plant model. Conservation of clock function is less explored in plants from the tropics. Cassava (Manihot esculenta) is a staple crop grown in the tropics that has been of limited research inter...


Adeyemo, O. SarahKolmos, ETohme, Joseph M.Chavarriaga Aguirre, PaulFregene, Martin A.Davis, S.J.[Identification and characterization of the Cassava core-clock gene Early Flowering 4]Identification and characterization of the Cassava core-clock gene Early Flowering 4

The importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. Plant biotechnology offers a wide range of opportunities that can help cassava become a b...


Chavarriaga Aguirre, PaulBrand, AlejandroMedina, AdrianaPrías, MónicaEscobar Pérez, Roosevelt H.Martínez, JuanDíaz, PaulaLópez Carrascal, Camilo ErnestoRoca, William M.Tohme, Joseph M.[The potential of using biotechnology to improve cassava: a review]The potential of using biotechnology to improve cassava: a review

Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping...


Chavarriaga Aguirre, PaulMaya, M.M.Bonierbale, Merideth W.Kresovich, SFregene, Martin A.Tohme, Joseph M.Kochert, G[Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability]Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability

Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accum...


Welsch, RArango, JacoboBar, CSalazar, BAl-Babili, S.Beltrán Giraldo, Jorge AlonsoChavarriaga Aguirre, PaulCeballos, H.Tohme, Joseph M.Beyer, Peter[Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W]]Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W]
Beltrán Giraldo, Jorge AlonsoJaimes, HEcheverry, MLadino Franco, YJLópez, D.Duque E., Myriam CristinaChavarriaga Aguirre, PaulTohme, Joseph M.[Quantitative analysis of transgenes in cassava plants using real-time PCR technology]Quantitative analysis of transgenes in cassava plants using real-time PCR technology

The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme p...


Chavarriaga Aguirre, PaulMaya, M.M.Tohme, Joseph M.Duque E., Myriam CristinaIglesias, C.A.Bonierbale, Merideth W.Kresovich, SKochert, G[Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections]Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections

Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledon...


Sarria, RATorres Castro, Edgar AlonsoAngel, F.Chavarriaga Aguirre, PaulRoca, William M.[Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation]Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation

A major constraint for incorporating new traits into cassava using biotechnology is the limited list of known/tested promoters that encourage the expression of transgenes in the cassava’s starchy roots. Based on a previous report on the glutamic-acid-rich protein Pt2L4, indicating a preferential ...


Beltrán Giraldo, Jorge AlonsoPrias, MAl-Babili, S.Ladino Franco, YJLópez, D.Beyer, PeterChavarriaga Aguirre, PaulTohme, Joseph M.[Expression pattern conferred by a glutamic acid-rich protein gene promoter in field-grown transgenic cassava (Manihot esculenta Crantz)]Expression pattern conferred by a glutamic acid-rich protein gene promoter in field-grown transgenic cassava (Manihot esculenta Crantz)

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