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Brachiaria species are widely sown on the infertile and Al-toxic soils of neotropical savannas. Breeding programs seek to combine edaphic adaptation with other traits in interspecific hybrids. Edaphic adaptation is difficult to assess because it is only manifest in pasture persistence across seve...


Wenzl, PeterArango Vélez, AdrianaChavez, Alba L.Buitrago González, M.E.Patino, GMMiles, John W.Rao, Idupulapati M.[A greenhouse method to screen brachiariagrass genotypes for aluminum resistance and root vigor]A greenhouse method to screen brachiariagrass genotypes for aluminum resistance and root vigor

Aluminum‐resistant Brachiaria decumbens Stapf cv. Basilisk (signalgrass) and closely related, but less resistant Brachiaria ruziziensis Germain & Evrard cv. Common (ruzigrass) both accumulated high concentrations of aluminum (Al) in roots. Approximately two thirds of the total Al was complexed by...


Wenzl, PeterChavez, Alba L.Patiño, Gloria M.Mayer, Jorge EdgardRao, Idupulapati M.[Aluminum stress stimulates the accumulation of organic acids in root apices of Brachiaria species]Aluminum stress stimulates the accumulation of organic acids in root apices of Brachiaria species

Application of field based high-throughput phenotyping (FB-HTP) methods for monitoring plant performance in real field conditions has a high potential to accelerate the breeding process. In this paper, we discuss the use of a simple tower based remote sensing platform using modified single-lens r...


Naito, HirokiOgawa, SatoshiValencia, Milton OrlandoMohri, HirokiUrano, YutakaHosoi, FumikiShimizu, YoChavez, Alba L.Ishitani, ManabuSelvaraj, Michael GomezOmasa, Kenji[Estimating rice yield related traits and quantitative trait loci analysis under different nitrogen treatments using a simple tower-based field phenotyping system with modified single-lens reflex cameras]Estimating rice yield related traits and quantitative trait loci analysis under different nitrogen treatments using a simple tower-based field phenotyping system with modified single-lens reflex cameras

The cassava core collection (601 genotypes) was evaluated for root and leaf contents of micronutrient minerals, ascorbic acid, and carotene. Wide genetic variability was observed for all measurements, indicating that there is good potential for exploiting and improving the nutritive value of cass...


Chavez, Alba L.Bedoya Flores, JMSánchez, T.Iglesias, C.A.Ceballos, H.Roca, William M.[Iron, carotene, and ascorbic acid in cassava roots and leaves]Iron, carotene, and ascorbic acid in cassava roots and leaves

Rapid-cycling recurrent selection has been implemented to produce cassava varieties that deliver provitamin A carotenoids to human populations that are affected by vitamin A deficiency. Rapid-cycling selection relies on quantification in just one plant per genotype. This study was conducted to a...


Ortiz, D.Sánchez, T.Morante, NCeballos, H.Pachón, HelenaDuque E., Myriam CristinaChavez, Alba L.Escobar Salamanca, Andrés F.[Sampling strategies for proper quantification of carotenoid content in cassava breeding]Sampling strategies for proper quantification of carotenoid content in cassava breeding

Post-harvest physiological deterioration (PPD) is one of the most important constraints in cassava production and commercialization. It has been hypothesized that the antioxidant properties of carotenoids in yellow cassava roots may help reduce or delay PPD. The industrial sector prefers cassava ...


Sánchez, T.Chavez, Alba L.Ceballos, H.Rodríguez Amaya, D.B.Nestel, PIshitani, Manabu[Reduction or delay of post-harvest physiological deterioration in cassava roots with higher carotenoid content]Reduction or delay of post-harvest physiological deterioration in cassava roots with higher carotenoid content

Processing of large numbers smaples of plant tissue samples for molecular mapping and gene tagging requires methods that are quick, simple, and cheap, and that eventually can be automated. Organic solvents used for DNA extraction can represent a significant proportion of the overall cost. In this...


Chavez, Alba L.Vergara, CEMayer, Jorge Edgard[Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues]Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues
Wenzl, PeterChavez, Alba L.Buitrago, María EugeniaPatiño, Gloria M.Miles, John W.Rao, Idupulapati M.[Development and validation of a hydroponic screening method to identify acid soil adapted genotypes of the tropical forage grass brachiaria]Development and validation of a hydroponic screening method to identify acid soil adapted genotypes of the tropical forage grass brachiaria

About 70 million people obtain more than 500 cal per day from cassava roots. The crop is fundamental as food security of poor rural communities, but little is known about variability of root nutritional and quality traits. Roots from 2457 genotypes comprising landraces and improved clones, were s...


Chavez, Alba L.Sánchez, T.Jaramillo, GustavoBedoya Flores, JMEcheverry, JBolaños Vidal, E.A.Ceballos, H.Iglesias, C.A.[Variation of quality traits in cassava roots evaluated in landraces and improved clones]Variation of quality traits in cassava roots evaluated in landraces and improved clones

Al resistance of signalgrass (Brachiaria decumbens Stapf cv Basilisk), a widely sown tropical forage grass, is outstanding compared with the closely related ruzigrass (Brachiaria ruziziensis Germain and Evrard cv Common) and Al-resistant genotypes of graminaceous crops such as wheat, triticale, a...


Wenzl, PeterPatino, GMChavez, Alba L.Mayer, Jorge EdgardRao, Idupulapati M.[The high level of aluminum resistance in signalgrass is not associated with known mechanism of external aluminum detoxification in root apices]The high level of aluminum resistance in signalgrass is not associated with known mechanism of external aluminum detoxification in root apices

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