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In this research we evaluated simple aspects of the sperm biology of Genypterus blacodes, in particular assessing the effects of pH (6, 7, 8 and 9), temperature (4, 8 and 16 degrees C) and osmolalities 100% sea water (1010 mOsm/kg, Control), 75% sea water (774 mOsm/kg, T-1), 50% sea water (488 mO...


Dumorne, KellyValdebenito, IvánContreras, PabloUlloa Rodríguez, PatricioRisopatrón, JennieFigueroa, ElíasEstévez, Manuel LeeDíaz, RommyFarías, Jorge[Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))]Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))

Sperm cryopreservation is an important tool for reproductive management; however, it has not been possible to perform this procedure for coho salmon. This study was designed to assess the effect of cryopreservation on sperm functional parameters, oxidative stress markers and fertilizing capacity ...


Sandoval Vargas, LeydyDumorne, KellyContreras, PabloFarias, Jorge G.Figueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, Iván[Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity]Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity

Storage of refrigerated semen is a simple and inexpensive procedure that can facilitate the management and reproduction programmes in aquaculture and allows to store semen for in vitro reproduction, quality analyses, hatchery production support, selective in vitro breeding, disease diagnosis, tra...


Contreras, PabloDumorne, KellyUlloa-Rodriguez, PatricioMerino, OsvaldoFigueroa Villalobos, ElíasFarias, Jorge G.Valdebenito Isler, IvánRisopatron, Jennie[Effects of short-term storage on sperm function in fish semen: a review]Effects of short-term storage on sperm function in fish semen: a review

Patagonian blenny (E. maclovinus) is a marine species recently placed in captivity and which are potentially farmable. Understanding and improving its sperm capacity to withstand short-term storage conditions is a key element of initiating an artificial propagation program for this species. The a...


Ulloa Rodriguez, PatricioContreras, PabloDumorne, KellyLee Estevez, ManuelDiaz, RommyFigueroa Villalobos, ElíasValdebenito Isler, IvánRisopatron, JennieFarias, Jorge G.[Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium]Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium

The development of semen cryopreservation strategies is necessary to improve the semen storage technologies of species of great commercial interest for aquaculture. Recent studies demonstrate that lipids play an important role in the fertility and cryotolerance of fish gametes. This study investi...


Diaz, RommyQuinones, JohnShort, StefaniaContreras, PabloUlloa Rodriguez, PatricioCancino Baier, DavidSepulveda, NestorValdebenito Isler, IvánFarias, Jorge G.[Effect of exogenous lipids on cryotolerance of Atlantic salmon (Salmo salar) spermatozoa]Effect of exogenous lipids on cryotolerance of Atlantic salmon (Salmo salar) spermatozoa

In this study, the morphology and ultrastructure of Eleginops maclovinus spermatozoa were characterized through scanning and electron microscopy. Findings revealed that E. maclovinus spermatozoa can be differentiated into three major parts: a spherical head without acrosome (typical for externall...


Ulloa Rodriguez, PatricioContreras, PabloDumorne, KellyFigueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, IvánFarias, Jorge G.[Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)]Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)
Sandoval Vargas, LeydyDumorne, KellyContreras, PabloFarias, Jorge G.Figueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, Iván[Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity (vol 533, 736151, 2021)]Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity (vol 533, 736151, 2021)
Dumorne, KellyValdebenito Isler, IvánContreras, PabloUlloa Rodriguez, PatricioRisopatron, JennieFigueroa Villalobos, ElíasEstevez, Manuel LeeDiaz, RommyFarias, Jorge[Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))]Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))

Calcium is a prerequisite factor for triggering the sperm activation in salmonids. The aim of this study was to test different concentrations of Ca(2+)on sperm motility activation and fertility of rainbow trout (Oncorhynchus mykiss). Semen samples activated with calcium free activator (0.0 mM) an...


Sandoval Vargas, LeydyFigueroa Villalobos, EliasContreras, PabloEstay, FranciscoValdebenito Isler, Iván[Effect of different calcium concentration on sperm motility and fertilisation capacity of rainbow trout (Oncorhynchus mykiss)]Effect of different calcium concentration on sperm motility and fertilisation capacity of rainbow trout (Oncorhynchus mykiss)

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