We have developed an immunochromatographic test for the diagnosis of peste des petits ruminants (PPR) under field conditions. The diagnostic assay has been tested in the laboratory and also under field conditions in Ivory Coast, Pakistan, Ethiopia and Uganda. The test is carried out on a superfic...
Baron, J.
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Fishbourne, E.
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Couacy-Hyman, E.
,
Abubakar, M.
,
Jones, B.A.
,
Frost, L.
,
Herbert, R.
,
Chibssa, T.R.
,
Klooster, G. van't
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Afzal, M.
,
Ayebazibwe, C.
,
Toye, Philip G.
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Bashiruddin, J.
,
Baron, M.D.
,
[Development and testing of a field diagnostic assay for peste des petits ruminants virus]
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Development and testing of a field diagnostic assay for peste des petits ruminants virus
A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the...
Fernández Pinero, J.
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Gallardo, C.
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Elizalde, M.
,
Robles, A.
,
Gómez, C.
,
Bishop, Richard P.
,
Heath, L.
,
Couacy-Hyman, E.
,
Fasina, F.O.
,
Pelayo, V.
,
Soler Garzón, Alvaro
,
Arias, Marisa
,
[Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library]
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Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library
The presence of antibodies against African swine fever (ASF), a complex fatal notifiable OIE disease of swine, is always indicative of previous infection, since there is no vaccine that is currently used in the field. The early appearance and subsequent long-term persistence of antibodies combine...
Gallardo, C.
,
Soler Garzón, Alvaro
,
Nieto, R.
,
Carrascosa, A.L.
,
Mia, G.M. de
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Bishop, Richard P.
,
Martins, C.
,
Fasina, F.O.
,
Couacy-Hyman, E.
,
Heath, L.
,
Pelayo, V.
,
Martin, E.
,
Simon, A.
,
Martin, R.
,
Okurut, A.R.
,
Lekolol, I.
,
Okoth, Edward A.
,
Arias, Marisa
,
[Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests]
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Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests