Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops. Several excision systems exist but none have been optimized or shown to...
Cuéllar, Wilmer J.
,
Gaudin, A.
,
Solorzano, D.
,
Casas, A.
,
Nopo, L.
,
Chudalayandi, P.
,
Medrano, G.
,
Kreuze, J.F.
,
Ghislain, M.
,
[Self-excision of the antibiotic resistance gene npII using a heat inducible Cre-loxP system from transgenic potato.]
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Self-excision of the antibiotic resistance gene npII using a heat inducible Cre-loxP system from transgenic potato.
Martínez, Angélica
,
Cuervo Ibáñez, Maritza
,
Aranzales Rondón, Ericson
,
Muñoz, Lucely
,
Carvajal Yepes, Mónica
,
Cuéllar, Wilmer J.
,
Debouck, Daniel G.
,
[Standardization and implementation of the RT-PCR methodology for the diagnostic of cassava viruses]
,
Standardization and implementation of the RT-PCR methodology for the diagnostic of cassava viruses
Olaya, Nubia
,
Betancourt, Monica
,
Cuéllar, Wilmer J.
,
Pappu, Hanu R.
,
[First Report of natural infection of tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum) by Alstroemeria necrotic streak virus in Colombia]
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First Report of natural infection of tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum) by Alstroemeria necrotic streak virus in Colombia
Souza Richards, R.
,
Adams, I.P.
,
Kreuze, J.F.
,
Souza, J. de
,
Cuéllar, Wilmer J.
,
Dullemans, A.M.
,
Vlugt, René A.A. van de
,
Glover, R.
,
Hany, U.
,
Dickinson, M.
,
Boonham, N.
,
[The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses.]
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The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses.
Vascular wilt of banana and plantains, also known as Moko disease, is caused by Ralstonia solanacearum (Rs) phylotype II, and is the main bacterial disease affecting these crops in the Americas. Upon comparative sequence analysis of 44 Rs genomes we developed an improved PCR protocol based on the...
Pardo, Juan Manuel
,
López-Alvarez, Diana
,
Ceballos, German
,
Álvarez, Elizabeth
,
Cuéllar, Wilmer J.
,
[Detection of Ralstonia solanacearum phylotype II, race 2 causing Moko disease and validation of genetic resistance observed in the hybrid plantain FHIA-21]
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Detection of Ralstonia solanacearum phylotype II, race 2 causing Moko disease and validation of genetic resistance observed in the hybrid plantain FHIA-21
Andean potato mottle virus (APMoV, genero Comovirus) es un patogeno importante de varias plantas Solanaceas, que incluyen papa, tomate y tabaco, pero hasta la fecha solo esta disponible datos de secuencias parciales. En este estudio, se determino el genoma completo de APMoV utilizando secuenciami...
Souza, J. de
,
Muller, G.
,
Cuéllar, Wilmer J.
,
Kreuze, J.F.
,
[Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.]
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Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.
El virus colusivo del camote (SPCV, genero Cavemovirus), el virus del aclaramiento de venas del camote (SPVC, genero Solendovirus) y el virus del enrollamiento de hojas del camote (SPLCV, genero Begomovirus) son virus con genoma de ADN, presentes en el camote en infecciones virales simples o mult...
Berrocal, A.
,
Rossel, G.
,
Fuentes, S.
,
Pérez, A.
,
Cuéllar, Wilmer J.
,
Kreuze, J.F.
,
[Desarrollo y aplicacion de PCR multiple para la deteccion simultanea de tres virus de ADN en camote (Ipomoea batatas L.)]
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Desarrollo y aplicacion de PCR multiple para la deteccion simultanea de tres virus de ADN en camote (Ipomoea batatas L.)
Los sweepovirus forman un grupo dentro del genero Begomovirus que se caracterizan por infectar solo a camote, los cuales comunmente causan infeccion asintomatica, pasando inadvertidos, por lo que su prevalencia y distribucion es desconocida en algunas regiones del mundo. Las infecciones multiples...
Galvez, M.
,
Cuéllar, Wilmer J.
,
Fuentes, S.
,
Kreuze, J.F.
,
[Caracterizacion molecular de aislamientos de sweepovirus que infectan Ipomoea batatas (L.) Lam. y estudio de sinergismo con el sweet potato chlorotic stunt virus (SPCSV).]
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Caracterizacion molecular de aislamientos de sweepovirus que infectan Ipomoea batatas (L.) Lam. y estudio de sinergismo con el sweet potato chlorotic stunt virus (SPCSV).
A place to integrate and collectively monitor the occurrence and movement of pests and diseases. It is based on symptoms recording and confirmation by molecular diagnostics
Cuéllar, Wilmer J.
,
Mwanzia, Leroy
,
Lourido, Derlyn
,
García, Carolina
,
Cruz, Paola
,
Pino, Lizbeth
,
Tohme, Joseph M.
,
[PestDisPlace; Monitoring the distribution of pests and diseases. Version 1.0]
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PestDisPlace; Monitoring the distribution of pests and diseases. Version 1.0
Kreuze, J.F.
,
Koening, R
,
Souza, J. de
,
Vetten, H.J.
,
Muller, G
,
Flores, B.
,
Ziebell, H
,
Cuéllar, Wilmer J.
,
[The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species]
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The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species