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Sweetpotato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most important pathogens of sweetpotato (Ipomoea batatas L.). It can reduce yields by 50% by itself and cause various synergistic disease complexes when co-infecting with other viruses, including swe...


Kreuze, J.F.Klein, I.S.Ghislain, M.Valkonen, J.P.T.Untiveros, M.Cuéllar, Wilmer J.Lajo, G.Cipriani, P.[RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus.]RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus.

Double-stranded RNA (dsRNA)-specific endonucleases belonging to RNase III classes 3 and 2 process dsRNA precursors to small interfering RNA (siRNA) or microRNA, respectively, thereby initiating and amplifying RNA silencing-based antiviral defense and gene regulation in eukaryotic cells. However, ...


Kreuze, J.F.Savenkov, E.I.Cuéllar, Wilmer J.Xiangdong LiValkonen, J.P.T.[Viral class 1 RNase III involved in suppression of RNA silencing.]Viral class 1 RNase III involved in suppression of RNA silencing.

Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops. Several excision systems exist but none have been optimized or shown to...


Cuéllar, Wilmer J.Gaudin, A.Solorzano, D.Casas, A.Nopo, L.Chudalayandi, P.Medrano, G.Kreuze, J.F.Ghislain, M.[Self-excision of the antibiotic resistance gene npII using a heat inducible Cre-loxP system from transgenic potato.]Self-excision of the antibiotic resistance gene npII using a heat inducible Cre-loxP system from transgenic potato.

Sweet potato chlorotic stunt virus (genus Crinivirus) belongs to the family Closteroviridae, members of which have a conserved overall genomic organization but are variable in gene content. In the bipartite criniviruses, heterogeneity is pronounced in the 3'-proximal region of RNA1, which in swee...


Cuéllar, Wilmer J.Tairo, F.Kreuze, J.F.Valkonen, J.P.T.[Analysis of gene content in sweet potato chlorotic stunt virus RNA1 reveals the presence of the p22 RNA silencing suppressor in only a few isolates: Implications for viral evolution and synergism.]Analysis of gene content in sweet potato chlorotic stunt virus RNA1 reveals the presence of the p22 RNA silencing suppressor in only a few isolates: Implications for viral evolution and synergism.

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