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Cuéllar, Wilmer J.Cruzado, R.Fuentes, S.Untiveros, M.Soto, M.Kreuze, J.F.[Sequence characterization of a Peruvian isolate of sweet potato chlorotic stunt virus: Further variability and a model for p22 acquisition.]Sequence characterization of a Peruvian isolate of sweet potato chlorotic stunt virus: Further variability and a model for p22 acquisition.

An inverted repeat construct corresponding to a segment of the potato leaf roll virus coat protein gene was created under control of a constitutive promoter and transferred into a transformation vector with a heat inducible Cre-loxP system to excise the nptII antibiotic resistance marker gene. Fi...


Orbegozo, JeanetteSolorzano, DennisCuéllar, Wilmer J.Bartolini, Maria LupeGhislain, M.Kreuze, J.F.[Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi]Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi

We describe here the complete genome of Rice hoja blanca tenuivirus. The sequenced isolate was obtained by insect vector transmission from a symptomatic rice sample grown in Colombia. Sequence data from the four RNA components were obtained by deep sequencing (Illumina), and infections were confi...


Jiménez Polo, JenyferCarvajal-Yepes, MonicaLeiva, Ana MariaCruz Gallego, MaribelRomero, Luz ElenaBolaños, Carmen A.Lozano, IvanCuéllar, Wilmer J.[Complete Genome Sequence of Rice hoja blanca tenuivirus Isolated from a Susceptible Rice Cultivar in Colombia]Complete Genome Sequence of Rice hoja blanca tenuivirus Isolated from a Susceptible Rice Cultivar in Colombia

Sweetpotato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most important pathogens of sweetpotato (Ipomoea batatas L.). It can reduce yields by 50% by itself and cause various synergistic disease complexes when co-infecting with other viruses, including swe...


Kreuze, J.F.Klein, I.S.Ghislain, M.Valkonen, J.P.T.Untiveros, M.Cuéllar, Wilmer J.Lajo, G.Cipriani, P.[RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus.]RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus.

Double-stranded RNA (dsRNA)-specific endonucleases belonging to RNase III classes 3 and 2 process dsRNA precursors to small interfering RNA (siRNA) or microRNA, respectively, thereby initiating and amplifying RNA silencing-based antiviral defense and gene regulation in eukaryotic cells. However, ...


Kreuze, J.F.Savenkov, E.I.Cuéllar, Wilmer J.Xiangdong LiValkonen, J.P.T.[Viral class 1 RNase III involved in suppression of RNA silencing.]Viral class 1 RNase III involved in suppression of RNA silencing.

Sweet potato (Ipomoea batatas) is an important subsistence and famine reserve crop grown in developing countries where Sweet potato chlorotic stunt virus (SPCSV; Closteroviridae), a single-stranded RNA (ssRNA) crinivirus, synergizes unrelated viruses in co-infected sweet potato plants. The most s...


Cuéllar, Wilmer J.Kreuze, J.F.Rajamaki, M.L.Cruzado, K.R.Untiveros, M.Valkonen, J.P.T.[Elimination of antiviral defense by viral RNase III.]Elimination of antiviral defense by viral RNase III.

A copy of the complete genome of a novel bipartite begomovirus infecting common bean (Phaseolus vulgaris L.) in Colombia was obtained by rolling-circle amplification (RCA), cloned, and sequenced. The virus is associated with leaf crumple symptoms and significant yield losses in Andean and Mesoame...


Carvajal-Yepes, MonicaZambrano, LeidyBueno, Juan MiguelRaatz, BodoCuéllar, Wilmer J.[Complete genome sequence of bean leaf crumple virus, a novel begomovirus infecting common bean in Colombia]Complete genome sequence of bean leaf crumple virus, a novel begomovirus infecting common bean in Colombia
Villamil-Garzon, AngelaCuéllar, Wilmer J.Guzmán-Barney, Mónica[Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia = Co-infección natural de Potato yellow vein virus y potivirus en cultivos de Solanum tuberosum en Colombia]Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia = Co-infección natural de Potato yellow vein virus y potivirus en cultivos de Solanum tuberosum en Colombia
Bolaños, Carmen A.Leiva Sandoval, Ana MariaSaavedra, JuanBruzzone, CarlosCruz Gallego, MaribelCuéllar, Wilmer J.[Occurrence and molecular detection of Rice hoja blanca virus (Genus Tenuivirus) in Peru]Occurrence and molecular detection of Rice hoja blanca virus (Genus Tenuivirus) in Peru

Cuéllar, Wilmer J.Galvez, MarcoFuentes, S.Tugume, Joab K.Kreuze, J.F.[Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)]Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)

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