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Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing se...


Wambugu, J.Kanguha, E.Hanotte, Olivier H.Davis, S.Taylor, J.Skow, L.Brunner, R.Schwerin, M.Teale, A.J.Iraqi, F.A.[Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone]Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone

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