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A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...
Heritability of susceptibility to infection with Theileria parva was estimated from full sib families of Rhipicephalus appendiculatus ticks. Male and female ticks of 2 stocks were mated singly. Nineteen full sib families of the Mugufa stock and 17 full sib families of the Kiambu stock were obtain...
Theileria parva (Boleni) stock from Zimbabwe was used to immunise 24 susceptible Friesian calves by infection and treatment with oxytetracycline. Twenty-eight days after immunisation the animals in groups of 6 plus 2 susceptible controls were subjected to challenge: 3 groups with heterologous sto...
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