Radopholus similisis a major pest of East African highland cooking bananas (Musaspp.) in Uganda. Non-pathogenic Fusariumoxysporumendophytes, isolated from bananas in farmers’ fields, have shown potential to reduceR. Similis numbers in tissue culture banana. The mechanism through which endophyte...
Paparu, P.
,
Dubois, T.
,
Coyne, D.L.
,
Viljoen, A.
,
[Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis]
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Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis
Two major biotic constraints to highland cooking
banana (Musa spp., genome group AAA-EA) production in
Uganda are the banana weevil Cosmopolites sordidus and
the burrowing nematode Radopholus similis. Endophytic
Fusarium oxysporum strains inoculated into tissue culture
banana plantlets have shown...
Paparu, P.
,
Dubois, T.
,
Gold, C.S.
,
Niere, B.
,
Adipala, E.
,
Coyne, D.L.
,
[Screenhouse and field persistence of nonpathogenic endophytic Fusarium oxysporum in Musa tissue culture plants]
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Screenhouse and field persistence of nonpathogenic endophytic Fusarium oxysporum in Musa tissue culture plants
Banana (Musa spp.) is a vegatatively propagated crop and the type of planting material is of great importancefor the productivity of banana plantations. Traditionally, sucker derived planting materials havebeen used to establish banana plantations but there is a risk of transmitting pests such as...
Niere, B.
,
Gold, C.
,
Coyne, D.L.
,
Dubois, T.
,
Sikora, R.A.
,
[Performance of tissuecultured versus suckerderived East African highland banana (Musa AAAEA) under high and low input systems in Uganda]
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Performance of tissuecultured versus suckerderived East African highland banana (Musa AAAEA) under high and low input systems in Uganda
Endophytic non-pathogenic Fusarium oxysporum isolates are known to control Radopholus similis through induced resistance. Using complementary (c)DNA-amplified fragment length polymorphism (cDNA-AFLP), we identified genes induced in nematode-susceptible and -tolerant bananas following colonization...
Paparu, P.
,
Dubois, T.
,
Coyne, D.L.
,
Viljoen, A.
,
[Differential gene expression in East African highland bananas (Musa spp.): interactions between nonpathogenic Fusarium V5w2 and Radopholus similis]
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Differential gene expression in East African highland bananas (Musa spp.): interactions between nonpathogenic Fusarium V5w2 and Radopholus similis
Among the major constriats to highland cooking banana (Musa spp. AAA-EA) production in Uganda is the high pests infestation levels. Most important are the banana weevil cosmopolites sordidus and a complex of plant parasitic root nematodes of which Radopholus similis is the most important. Clean p...
Paparu, P.
,
Dubois, T.
,
Athman, S.
,
Kapindu, S.
,
Coyne, D.
,
Gold, C.
,
Adipala, E.
,
Mukwaba, E.
,
[Merging biotechnology with biological control: banana Musa tissue culture plants enhanced by endophytic fungi]
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Merging biotechnology with biological control: banana Musa tissue culture plants enhanced by endophytic fungi
In studying plant colonization by inoculated Fusarium oxysporum endophytes, it is important to be able to distinguish inoculated isolates from saprophytic strains. In the current study, F. oxysporum isolates were transformed with the green (GFP) and red fluorescent protein (DsRed) genes, and beno...
Paparu, P.
,
Macleod, A.
,
Dubois, T.
,
Coyne, D.L.
,
Viljoen, A.
,
[Efficacy of chemical and flourescent protein markers in studying plant colonization by endophytic nonpathogenic Fusarium oxysporum isolates]
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Efficacy of chemical and flourescent protein markers in studying plant colonization by endophytic nonpathogenic Fusarium oxysporum isolates
We studied the effect of treating banana and plantain (Musa spp.) suckers using boiling water for the generation of healthy planting material. Small, medium and large-sized suckers of two East African highland bananas (cvs. Mbwazirume, Kibuzi, AAA-EA) and one of plantain (cv. Agbagba, AAB) were d...
Coyne, D.L.
,
Wasukira, A.
,
Dusabe, J.
,
Rotifa, I.
,
Dubois, T.
,
[Boiling water treatment: a simple, rapid and effective technique for nematode and banana weevil management in banana and plantain (Musa spp.) planting material]
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Boiling water treatment: a simple, rapid and effective technique for nematode and banana weevil management in banana and plantain (Musa spp.) planting material
Banana (Musaspp.) in East Africa is threatened by pests and diseases, of which the burrowingnematode, Radopholus similis, is among the most serious and difficult to control. Fungal antagonists, such as Paecilomyces spp., can aid in controlling R. similis. In this study, Paecilomyces spp. was isol...
El cultivo de banano (Musaspp.) en Africa Oriental se ve afectado por plagas y enfermedades, en-tre las cuales el nematodo barrenador, Radopholus similis,es uno de los más serios y difíciles de con-trolar. Los hongos antagonistas, como Paecilomycesspp., pueden ayudar a controlar R. similis. En es...
Kilama, P.
,
Dubois, T.
,
Coyne, D.L.
,
Niere, B.
,
Gold, C.S.
,
Adipala, E.
,
[Antagonism of Paecilomyces spp. isolated from banana (Musa spp.) roots and rhizosphere against Radopholus similis]
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Antagonism of Paecilomyces spp. isolated from banana (Musa spp.) roots and rhizosphere against Radopholus similis
Akello, J.
,
Coyne, D.L.
,
Wasukira, A.
,
Dubois, T.
,
[Endophytic Beauvaria bassiana controls: Radopholus similis in tissue culture bananas]
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Endophytic Beauvaria bassiana controls: Radopholus similis in tissue culture bananas
The effects of nematode infection and mulching on plantain cv. Agbagba (Musa spp., AAB-group, false horn) yield and plantation longevity were examined in a field experiment at the High Rainfall Station of the International Institute of Tropical Agriculture (IITA) at Onne in southeastern Nigeria. ...
Coyne, D.L.
,
Rotimi, O.
,
Speijer, P.
,
Schutter, B. de
,
Dubois, T.
,
Auwerkerken, A.
,
Tenkouano, A.
,
Waele, D. de
,
[Effects of nematode infection and mulching on the yield of plantain (Musa spp., AABgroup) ratoon crops and plantation longevity in Southeastern Nigeria]
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Effects of nematode infection and mulching on the yield of plantain (Musa spp., AABgroup) ratoon crops and plantation longevity in Southeastern Nigeria