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In this research we evaluated simple aspects of the sperm biology of Genypterus blacodes, in particular assessing the effects of pH (6, 7, 8 and 9), temperature (4, 8 and 16 degrees C) and osmolalities 100% sea water (1010 mOsm/kg, Control), 75% sea water (774 mOsm/kg, T-1), 50% sea water (488 mO...


Dumorne, KellyValdebenito, IvánContreras, PabloUlloa Rodríguez, PatricioRisopatrón, JennieFigueroa, ElíasEstévez, Manuel LeeDíaz, RommyFarías, Jorge[Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))]Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))

Sperm cryopreservation is an important tool for reproductive management; however, it has not been possible to perform this procedure for coho salmon. This study was designed to assess the effect of cryopreservation on sperm functional parameters, oxidative stress markers and fertilizing capacity ...


Sandoval Vargas, LeydyDumorne, KellyContreras, PabloFarias, Jorge G.Figueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, Iván[Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity]Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity

Storage of refrigerated semen is a simple and inexpensive procedure that can facilitate the management and reproduction programmes in aquaculture and allows to store semen for in vitro reproduction, quality analyses, hatchery production support, selective in vitro breeding, disease diagnosis, tra...


Contreras, PabloDumorne, KellyUlloa-Rodriguez, PatricioMerino, OsvaldoFigueroa Villalobos, ElíasFarias, Jorge G.Valdebenito Isler, IvánRisopatron, Jennie[Effects of short-term storage on sperm function in fish semen: a review]Effects of short-term storage on sperm function in fish semen: a review

Patagonian blenny (E. maclovinus) is a marine species recently placed in captivity and which are potentially farmable. Understanding and improving its sperm capacity to withstand short-term storage conditions is a key element of initiating an artificial propagation program for this species. The a...


Ulloa Rodriguez, PatricioContreras, PabloDumorne, KellyLee Estevez, ManuelDiaz, RommyFigueroa Villalobos, ElíasValdebenito Isler, IvánRisopatron, JennieFarias, Jorge G.[Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium]Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium

Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...


Dumorne, KellyFigueroa Villalobos, ElíasCosson, JackyLee Estevez, ManuelUlloa Rodriguez, PatricioValdebenito Isler, IvánFarias, Jorge G.[Protein phosphorylation and ions effects on salmonid sperm motility activation]Protein phosphorylation and ions effects on salmonid sperm motility activation

The main objective of this work was to study the effects of banana flour as energy sources on broiler performance. Seventy-five broilers were randomly distributed into five groups each with 15 broilers (n = 15 broilers/group). The broilers were grouped to maize-soybean meal diet as control, T-1: ...


Dumorne, KellyAstorga-Elo, MarciaMerino, OsvaldoSevere, RolordMorante, Laura[Importance of banana flour and its effect on growth performance of broiler]Importance of banana flour and its effect on growth performance of broiler

In this study, the morphology and ultrastructure of Eleginops maclovinus spermatozoa were characterized through scanning and electron microscopy. Findings revealed that E. maclovinus spermatozoa can be differentiated into three major parts: a spherical head without acrosome (typical for externall...


Ulloa Rodriguez, PatricioContreras, PabloDumorne, KellyFigueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, IvánFarias, Jorge G.[Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)]Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...


Lee Estevez, ManuelHerrera, LisandraDiaz, RommyBeltran, JorgeFigueroa Villalobos, ElíasDumorne, KellyUlloa Rodriguez, PatricioShort, StefaniaRisopatron, JennieValdebenito Isler, IvánFarias, Jorge[Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility]Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility

The aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (Delta Psi Mit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into thre...


Bravo, WilliamsDumorne, KellyBeltran Lissabet, JorgeJara Seguel, PedroRomero, JaimeFarias, Jorge G.Risopatron, JennieValdebenito Isler, IvánFigueroa Villalobos, Elías[Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa]Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa

The development of sperm cryopreservation has enabled transcendental changes to occur in the reproductive biotechnology of both mammals and fish; it has become a basic tool for animal improvement. Nevertheless, these protocols cause damage to cell structure and physiology, altering sperm function...


Figueroa Villalobos, ElíasValdebenito Isler, IvánZepeda, Andrea B.Figueroa, Carolina A.Dumorne, KellyCastillo, Rodrigo L.Farias, Jorge G.[Effects of cryopreservation on mitochondria of fish spermatozoa]Effects of cryopreservation on mitochondria of fish spermatozoa

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